Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies
DescriptionGoat polyclonal to Clathrin light chain
Specificityab129326 is expected to recognize all reported isoforms of light chains a and b.
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Gorilla
- Human Spleen tissue; Human Frontal Cortex lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab129326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.1 - 0.3 µg/ml.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles.
- Information by UniProt
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
ab129326, at 2.5 µg/ml, staining Clathrin light chain in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry.
Anti-Clathrin light chain antibody (ab129326) at 0.1 µg/ml + Human Frontal Cortex lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 23 kDa
Primary incubation was 1 hour.
ab129326 has not yet been referenced specifically in any publications.