Overview

  • Product name

    Anti-Clathrin light chain antibody
    See all Clathrin light chain primary antibodies
  • Description

    Goat polyclonal to Clathrin light chain
  • Host species

    Goat
  • Specificity

    ab129326 is expected to recognize all reported isoforms of light chains a and b.
  • Tested applications

    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Gorilla
  • Immunogen

    Synthetic peptide:

    CDFNPKSSKQAKD

    , corresponding to internal sequence amino acids 218-230 of Human Clathrin light chain a (NP_009027.1).

  • Positive control

    • Human Spleen tissue; Human Frontal Cortex lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab129326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 0.1 - 0.3 µg/ml.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 23, 35 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • ab129326, at 2.5 µg/ml, staining Clathrin light chain in formalin-fixed, paraffin-embedded Human spleen tissue by Immunohistochemistry.
  • Anti-Clathrin light chain antibody (ab129326) at 0.1 µg/ml + Human Frontal Cortex lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 23 kDa



    Primary incubation was 1 hour.

References

ab129326 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab129326.
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