Key features and details
- Goat polyclonal to Clathrin light chain
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Clathrin light chain antibody
See all Clathrin light chain primary antibodies
DescriptionGoat polyclonal to Clathrin light chain
SpecificityExpected to recognize all reported isoforms of light chains a and b.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Macaque monkey
- Recombinant Human Clathrin light chain protein (ab126693) can be used as a positive control in WB. Human frontal cortex lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation, followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab77542 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 23 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionClathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Cellular localizationCytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles.
- Information by UniProt
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
Anti-Clathrin light chain antibody (ab77542) at 0.1 µg/ml + Human frontal cortex lysate in RIPA buffer at 35 µg
Predicted band size: 23 kDa
Observed band size: 23 kDa
Additional bands at: 35 kDa (possible isoform)
The observed molecular weight of ~35kDa corresponds to earlier findings in literature with different antibodies (Okamoto et al, Am J Physiol Cell Physiol. 2000 Sep;279(3):C833-51.; PMID: 10942733)
ab77542 has been referenced in 1 publication.
- Liu H et al. Clathrin-mediated endocytosis in living host cells visualized through quantum dot labeling of infectious hematopoietic necrosis virus. J Virol 85:6252-62 (2011). WB . PubMed: 21525360