Overview

  • Product name

    Anti-Claudin 1 antibody [CL3698]
    See all Claudin 1 primary antibodies
  • Description

    Mouse monoclonal [CL3698] to Claudin 1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human Claudin 1 aa 189-211.
    Sequence:

    KTTSYPTPRPYPKPAPSSGKDYV


    Database link: O95832

  • Positive control

    • IHC-P: Human liver, small intestine, placenta, prostate, prostate cancer, breast cancer, liver cancer and colorectal cancer tissue. WB: RT4 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituents: 40% Glycerol, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from TCS.
  • Clonality

    Monoclonal
  • Clone number

    CL3698
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab242370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml.
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
    (Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
  • Tissue specificity

    Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
  • Involvement in disease

    Ichthyosis-sclerosing cholangitis neonatal syndrome
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction, tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Claudin-1 antibody
    • Claudin1 antibody
    • CLD 1 antibody
    • CLD1 antibody
    • CLD1_HUMAN antibody
    • CLDN 1 antibody
    • Cldn1 antibody
    • ILVASC antibody
    • SEMP 1 antibody
    • SEMP1 antibody
    • Senescence associated epithelial membrane protein 1 antibody
    • Senescence associated epithelial membrane protein antibody
    • Senescence-associated epithelial membrane protein antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • All lanes : Anti-Claudin 1 antibody [CL3698] (ab242370) at 1 µg/ml

    Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
    Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
  • Formalin-fixed, paraffin-embedded human placenta tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Negative control - Formalin-fixed, paraffin-embedded human lymph node tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver cancer tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human prostate tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human small intestine tissue stained for Claudin 1 with ab242370 at a 1:50 dilution in immunohistochemical analysis.

References

ab242370 has not yet been referenced specifically in any publications.

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