Recombinant Anti-Claudin 1 antibody [EPR9306] (ab180158)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9306] to Claudin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Claudin 1 antibody [EPR9306]
See all Claudin 1 primary antibodies -
Description
Rabbit monoclonal [EPR9306] to Claudin 1 -
Host species
Rabbit -
Specificity
Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096,PMID: 15965503).
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Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431, Human skin and fetal liver lysates, Mouse kidney and liver tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9306 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180158 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/2000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa).
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 19 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594). -
Tissue specificity
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. -
Involvement in disease
Ichthyosis-sclerosing cholangitis neonatal syndrome -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction, tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
- SwissProt: P56745 Rat
- Unigene: 439060 Human
see all -
Alternative names
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
see all
Images
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All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CLDN1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 23 kDaLanes 1 - 3: Merged signal (red and green). Green - ab180158 observed at 18 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab180158 was shown to specifically react with in wild-type A431 WT cells as signal was lost in A431 CLDN1 knockout cells. Wild-type and A431 CLDN1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3pc Milk. Ab180158 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/2000 dilution + Human fetal liver lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 23 kDa -
All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/10000 dilution
Lane 1 : A431 cell lysate
Lane 2 : Human skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 23 kDa -
All lanes : Anti-Claudin 1 antibody [EPR9306] (ab180158) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Mouse colon tissue lysate
Lane 4 : Mouse small intestine tissue lysate
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 60 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Claudin-1 expression is minimal in normal colon tissues and is increased in tumors (PMID: 17308096, PMID: 15965503).
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (23)
ab180158 has been referenced in 23 publications.
- Zheng H et al. Cordyceps militaris Modulates Intestinal Barrier Function and Gut Microbiota in a Pig Model. Front Microbiol 13:810230 (2022). PubMed: 35369439
- Wang R et al. Gut microbiota regulates acute myeloid leukaemia via alteration of intestinal barrier function mediated by butyrate. Nat Commun 13:2522 (2022). PubMed: 35534496
- Guo W et al. Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota. Foods 11:N/A (2022). PubMed: 35681301
- Feng J et al. Marine Chitooligosaccharide Alters Intestinal Flora Structure and Regulates Hepatic Inflammatory Response to Influence Nonalcoholic Fatty Liver Disease. Mar Drugs 20:N/A (2022). PubMed: 35736186
- Zhou X et al. Intestinal accumulation of microbiota-produced succinate caused by loss of microRNAs leads to diarrhea in weanling piglets. Gut Microbes 14:2091369 (2022). PubMed: 35758253