Recombinant Anti-Claudin 1 antibody [SP128] (ab115783)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP128] to Claudin 1
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Claudin 1 antibody [SP128]
See all Claudin 1 primary antibodies -
Description
Rabbit monoclonal [SP128] to Claudin 1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human liver carcinoma tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP128 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt |
1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594). -
Tissue specificity
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis. -
Involvement in disease
Ichthyosis-sclerosing cholangitis neonatal syndrome -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction, tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 414922 Cow
- Entrez Gene: 9076 Human
- Entrez Gene: 12737 Mouse
- Entrez Gene: 65129 Rat
- Omim: 603718 Human
- SwissProt: Q6L708 Cow
- SwissProt: O95832 Human
- SwissProt: O88551 Mouse
see all -
Alternative names
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab115783 has not yet been referenced specifically in any publications.