Product nameAnti-Claudin 1 antibody [SP128] - BSA and Azide free
See all Claudin 1 primary antibodies
DescriptionRabbit monoclonal [SP128] to Claudin 1 - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig
Synthetic peptide within Human Claudin 1 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95832
- Flow cyt: HepG2 cells. IHC-P: Human liver carcinoma tissue.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact email@example.com./span/strong
This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This antibody is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.??
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab271884 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
FunctionClaudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).
(Microbial infection) Acts as a receptor for hepatitis C virus in hepatocytes (PubMed:17325668). Acts as a receptor for dengue virus (PubMed:24074594).
Tissue specificityStrongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.
Involvement in diseaseIchthyosis-sclerosing cholangitis neonatal syndrome
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction, tight junction. Cell membrane.
- Information by UniProt
- Claudin-1 antibody
- Claudin1 antibody
- CLD 1 antibody
Flow cytometric analysis of rabbit anti-Claudin 1 (SP128) antibody ab115783 (1/100) in HepG2 cells (green) compared to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab115783).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab271884 has not yet been referenced specifically in any publications.