Product nameAnti-Claudin 10 antibody
See all Claudin 10 primary antibodies
DescriptionRabbit polyclonal to Claudin 10
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide (Human)
- Extracts from LOVO cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52234 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).|
|IHC-P||Use at an assay dependent concentration.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- Claudin-10 antibody
- Claudin10 antibody
- CLD10_HUMAN antibody
All lanes : Anti-Claudin 10 antibody (ab52234) at 1/300 dilution
Lane 1 : Extracts from LOVO cells, untreated.
Lane 2 : Extracts from LOVO cells, treated with the immunising peptide.
Predicted band size: 24 kDa
Observed band size: 24 kDa
ab52234 staining Claudin 10 in sections of Human proximal tubular cells by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in methanol prior to blocking with 5% BSA for 30 minutess at 20°C. The primary antibody was diluted 1/300 in 0.5% BSA/0.1% Triton X-100 and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 488-conjugated Donkey anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/800.
This product has been referenced in:
- Tang J et al. Effects of mesenchymal stem cells on interleukin-1ß-treated chondrocytes and cartilage in a rat osteoarthritic model. Mol Med Rep 12:1753-60 (2015). WB . Read more (PubMed: 25892273) »
- Aschauer L et al. Delineation of the key aspects in the regulation of epithelial monolayer formation. Mol Cell Biol 33:2535-50 (2013). Read more (PubMed: 23608536) »