Anti-Claudin 15 antibody (ab215354)
Key features and details
- Rabbit polyclonal to Claudin 15
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Claudin 15 antibody
See all Claudin 15 primary antibodies -
Description
Rabbit polyclonal to Claudin 15 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Claudin 15. The exact sequence is proprietary.
Database link: P56746 -
Positive control
- WB - K562, 231 and PC3 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 24 kDa.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 24 kDa. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 24146 Human
- SwissProt: P56746 Human
- Unigene: 38738 Human
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Alternative names
- Claudin-15 antibody
- CLD15_HUMAN antibody
- CLDN15 antibody
Images
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All lanes : Anti-Claudin 15 antibody (ab215354) at 1/200 dilution
Lane 1 : K562 cell lysate
Lane 2 : 231 cell lysate
Lane 3 : PC3 cell lysate
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Goat anti rabbit IgG at 1/8000 dilution
Developed using the ECL technique.
Predicted band size: 24 kDa
Exposure time: 1 minuteGel: 12%SDS-PAGE
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab215354 has been referenced in 1 publication.
- Guo W et al. Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate. Int J Oral Sci 10:e8 (2018). PubMed: 29319048