Overview

  • Product name

  • Description

    Rabbit polyclonal to Claudin 15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Claudin 15. The exact sequence is proprietary.
    Database link: P56746

  • Positive control

    • WB - K562, 231 and PC3 cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Preservative: 0.05% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab215354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/1000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Claudin-15 antibody
    • CLD15_HUMAN antibody
    • CLDN15 antibody

Images

  • All lanes : Anti-Claudin 15 antibody (ab215354) at 1/200 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : 231 cell lysate
    Lane 3 : PC3 cell lysate

    Lysates/proteins at 40 µg per lane.

    Secondary
    All lanes : Goat anti rabbit IgG at 1/8000 dilution

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 1 minute


    Gel: 12%SDS-PAGE

References

This product has been referenced in:

  • Guo W  et al. Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate. Int J Oral Sci 10:e8 (2018). Read more (PubMed: 29319048) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab215354.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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