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Mouse polyclonal to Claudin 15
Full length protein, corresponding to amino acids 1-228 of Human Claudin 15
Transfected 293T cell lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Constituents: 1X PBS, pH 7.2
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 24 kDa).
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Belongs to the claudin family.
Cell junction > tight junction. Cell membrane.
Information by UniProt
Western blot - Anti-Claudin 15 antibody (ab68631)
All lanes :
Anti-Claudin 15 antibody (ab68631) at 1/500 dilution
Lane 1 :
transfected 293T cell lysate
Lane 2 :
non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size:
Observed band size:
18 kDa (
why is the actual band size different from the predicted?
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"