Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    A region within synthetic peptide: GSNERAKAYL LGTSGVLFIL TGIFVLIPVS WTANIIIRDF YNPAIHIGQK, corresponding to internal sequence amino acids 109-158 of Human Claudin 17

  • Positive control
    • Jurkat cell lysate for western blot and human lung and spleen for immunohistochemistry.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    This antibody was purified by chromatography. 
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 25 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    Claudin proteins are a family of transmembrane proteins associated with tight junctions, and different tissues exhibit different Claudin composition. There are 18 homolgs present in humans, and mutations in any of these proteins cause a variety of human disease, this indicates their physiological importance. CLDN17, clustered with CLDN8 at human chromosome 21q22.11, is a four transmembrane protein with WWCC motif, defined by W-X(17-22)-W-X(2)-C-X(8-10)-C.
  • Cellular localization
    Integral membrane protein. Tight junctions.
  • Database links
  • Alternative names
    • CLDN17 antibody
    • Human CLDN17 gene for claudin 17 antibody

Images

  • Anti-Claudin 17 antibody (ab23333) at 2 µg/ml + Jurkat cell lysate (10ug)

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50,000 - 1/100,000 dilution.

    Predicted band size: 25 kDa
    Observed band size: 30 kDa
    why is the actual band size different from the predicted?

  • Paraffin-embedded sections of Human spleen cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Cluadin 17 are indicated by arrows. 400x magnification.

  • Paraffin-embedded sections of Human alveolar cells incubated with ab23333. The antibody was used at a range of 4.0-8.0µg/ml. Cells stained positive for Claudin 17 are indicated by arrows. 400x magnification.

References

This product has been referenced in:
  • Gao M  et al. The distinct expression patterns of claudin-10, -14, -17 and E-cadherin between adjacent non-neoplastic tissues and gastric cancer tissues. Diagn Pathol 8:205 (2013). Human . Read more (PubMed: 24325792) »
  • Farwick M  et al. Fifty-kDa hyaluronic acid upregulates some epidermal genes without changing TNF-a expression in reconstituted epidermis. Skin Pharmacol Physiol 24:210-7 (2011). WB ; Human . Read more (PubMed: 21412035) »
See all 3 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for your reply.
I am sorry to hear that the tips did not help to improve the results.
Since the antibody is being used in an untested application (ICC/IF), our Abpromise guarantee does not apply (thus I cannot refund the antibody). However, you can use our Abtrial program for untested species and applications as follows.

For UNTESTED species and/or applications, we have established a testing discount program. Here is a brief description of how it works:
The testing discount program is for customers who like to use an antibody/protein/kit on an untested species/application. You would purchase the to-be-tested product at full price, test it and submit an Abreview with your data (positive or negative). On your next order you will receive a discount in the amount of the price you paid for the to-be-tested product. The terms and conditions applicable to this offer can be found here: https://www.abcam.com/abtrial.
Since you already have submitted the Abreview for ICC/IF, you can go ahead and apply the discount code below to your next order:

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx
I am very pleased to hear you would like to accept our offer and test ab23333 in ICC/IF. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Read More

Answer

Thank you for submitting an Abreview for ab23333. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.
Based on the information in your review, I feel that altering the following steps in your protocol may help improve your results:
- blocking less long (e.g. 1h)
- trying a dfferent fixation method (e.g. PFA, acetone)
- using more primary antibody (1/100)
- incubating longer with the primary antibody (overnight 4 degree)
- checking that the secondary antibody is working well with other primaries.
If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney)
Specification
kidney
Fixative
Methanol
Permeabilization
Yes - Triton X
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 12 2012

Answer

Thank you for getting back to me. Generally if an immunogen forms an alignment of 6 or more consecutive residues I would consider it possible that the antibody would react against the homolog of the species. However, if it is a polyclonal antisera this is not always the case as given its polyclonal nature it is a mixture of immunoglobulin molecules. I would be very interested to know the results of your sequence comparisons as I could not find an immunogen that carried significant sequence similarity to warrant the suggestion of the application of the antibody to fish. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. I have performed a sequence analysis of your fish sequence with all of immunogens used to raise our antibodies against Claudin. Unfortunately none had significant sequence similarity that would make me consider reactivity likely. I am sorry that I cannot be of more assistance on this occasion.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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