Anti-Claudin 2 antibody (ab53032)
- Datasheet
- References (15)
- Protocols
Overview
-
Product nameAnti-Claudin 2 antibody
See all Claudin 2 primary antibodies -
DescriptionRabbit polyclonal to Claudin 2
-
Host speciesRabbit
-
Specificityab53032 detects endogenous levels of total Claudin 2 protein.
-
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
-
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
-
Immunogen
A synthetic peptide derived from human Claudin 2.
-
Positive control
- NIH 3T3 cell extracts.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
-
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab53032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| ELISA | 1/5000. |
Target
-
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
-
Sequence similaritiesBelongs to the claudin family.
-
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 9075 Human
- Entrez Gene: 12738 Mouse
- Entrez Gene: 300920 Rat
- Omim: 300520 Human
- SwissProt: P57739 Human
- SwissProt: O88552 Mouse
- Unigene: 522746 Human
- Unigene: 117068 Mouse
see all -
Alternative names
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 2 antibody (ab53032)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab53032. The picture on the right is blocked with the synthesized peptide.
-
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 2 antibody (ab53032)ICC/IF image of ab53032 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Claudin 2 antibody (ab53032)All lanes : Anti-Claudin 2 antibody (ab53032)
Lane 1 : NIH/3T3 cells
Lane 2 : NIH/3T3 cells with blocking peptide
Predicted band size: 25 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sun L et al. Increased expression of claudin-12 promotes the metastatic phenotype of human bronchial epithelial cells and is associated with poor prognosis in lung squamous cell carcinoma. Exp Ther Med 17:165-174 (2019). Read more (PubMed: 30651778) »
- Guo P et al. Effects of imipenem combined with low-dose cyclophosphamide on the intestinal barrier in septic rats. Exp Ther Med 16:1919-1927 (2018). Read more (PubMed: 30186419) »
