Anti-Claudin 2 antibody (ab53032)
- Datasheet
- References (22)
- Protocols
Overview
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Product name
Anti-Claudin 2 antibody
See all Claudin 2 primary antibodies -
Description
Rabbit polyclonal to Claudin 2 -
Host species
Rabbit -
Specificity
ab53032 detects endogenous levels of total Claudin 2 protein. -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
A synthetic peptide derived from human Claudin 2.
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Positive control
- NIH 3T3 cell extracts.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab53032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| ELISA | 1/5000. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9075 Human
- Entrez Gene: 12738 Mouse
- Entrez Gene: 300920 Rat
- Omim: 300520 Human
- SwissProt: P57739 Human
- SwissProt: O88552 Mouse
- Unigene: 522746 Human
- Unigene: 117068 Mouse
see all -
Alternative names
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 2 antibody (ab53032)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab53032. The picture on the right is blocked with the synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 2 antibody (ab53032)ICC/IF image of ab53032 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Claudin 2 antibody (ab53032)All lanes : Anti-Claudin 2 antibody (ab53032)
Lane 1 : NIH/3T3 cells
Lane 2 : NIH/3T3 cells with blocking peptide
Predicted band size: 25 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sun L et al. Increased expression of claudin-12 promotes the metastatic phenotype of human bronchial epithelial cells and is associated with poor prognosis in lung squamous cell carcinoma. Exp Ther Med 17:165-174 (2019). Read more (PubMed: 30651778) »
- Kiyohara H et al. Toll-Like Receptor 7 Agonist-Induced Dermatitis Causes Severe Dextran Sulfate Sodium Colitis by Altering the Gut Microbiome and Immune Cells. Cell Mol Gastroenterol Hepatol 7:135-156 (2019). Read more (PubMed: 30510995) »
