- Datasheet
- Specific References (9)
- Protocols
Overview
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Product nameAnti-Claudin 2 antibody
See all Claudin 2 primary antibodies -
DescriptionRabbit polyclonal to Claudin 2
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Host speciesRabbit
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Specificityab53032 detects endogenous levels of total Claudin 2 protein.
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Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISAmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
A synthetic peptide derived from human Claudin 2.
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Positive control
- NIH 3T3 cell extracts.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab53032 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab53032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). | |
| ELISA | 1/5000. |
Target
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FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
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Sequence similaritiesBelongs to the claudin family.
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Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 9075 Human
- Entrez Gene: 12738 Mouse
- Entrez Gene: 300920 Rat
- Omim: 300520 Human
- SwissProt: P57739 Human
- SwissProt: O88552 Mouse
- Unigene: 522746 Human
- Unigene: 117068 Mouse
see all -
Alternative names
- Claudin-2 antibody
- Claudin2 antibody
- CLD2_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 2 antibody (ab53032)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using ab53032. The picture on the right is blocked with the synthesized peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 2 antibody (ab53032)ICC/IF image of ab53032 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53032, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Western blot - Anti-Claudin 2 antibody (ab53032)All lanes : Anti-Claudin 2 antibody (ab53032) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract
Lane 2 : NIH 3T3 cell extract with immunising peptide
Predicted band size: 25 kDa
Observed band size: 25 kDa
Protocols
References
This product has been referenced in:
- JanssenDuijghuijsen LM et al. Mitochondrial ATP Depletion Disrupts Caco-2 Monolayer Integrity and Internalizes Claudin 7. Front Physiol 8:794 (2017). Read more (PubMed: 29075202) »
- Tsai PY et al. IL-22 Upregulates Epithelial Claudin-2 to Drive Diarrhea and Enteric Pathogen Clearance. Cell Host Microbe 21:671-681.e4 (2017). Read more (PubMed: 28618266) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"