Key features and details
- Rabbit polyclonal to Claudin 20
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Claudin 20 antibody
DescriptionRabbit polyclonal to Claudin 20
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
- WB: A549, HepG2 and PC-3 whole cell lysates. ICC/IF: HepG2 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab236726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 24 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- Claudin-20 antibody
- CLD20_HUMAN antibody
- CLDN20 antibody
All lanes : Anti-Claudin 20 antibody (ab236726) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 24 kDa
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Claudin 20 (green) using ab236726 at 1/100 dilution in ICC/IF, folowed by Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236726 has not yet been referenced specifically in any publications.