Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Claudin 3 antibody
See all Claudin 3 primary antibodies
DescriptionRabbit polyclonal to Claudin 3
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Claudin 3 (C terminal).
Database link: O15551
- Human small intestine; HuvEc cell extracts, HuvEc cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab133187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 23 kDa.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||1/100 - 1/500.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
All lanes : Anti-Claudin 3 antibody (ab133187) at 1/500 dilution
Lane 1 : Extracts of untreated HuvEc cells
Lane 2 : Extracts of HuvEc cells treated with the synthesized peptide
Predicted band size: 23 kDa
Immunohistochemical analysis of Claudin 3 in formalin fixed, paraffin embedded Human small intestine tissue stained with ab133187 at 10 µg/ml, followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Immunofluorescence analysis of Claudin 3 in HuvEc cells stained with ab133187 at a 1/100 dilution. Right panel represent cells treated with the synthesized peptide.
ab133187 has not yet been referenced specifically in any publications.