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  1. Link

    claudin-3-antibody-ab15102.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Tight Junctions
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Anti-Claudin 3 antibody (ab15102)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (51)

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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)

Key features and details

  • Rabbit polyclonal to Claudin 3
  • Suitable for: ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Chicken, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Claudin 3 antibody [EPR19971] (ab214487)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Claudin 3 antibody
    See all Claudin 3 primary antibodies
  • Description

    Rabbit polyclonal to Claudin 3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Chicken, Human, Pig
  • Immunogen

    Synthetic peptide within Mouse Claudin 3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q9Z0G9

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Tight Junctions
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Claudin 3 protein (ab114332)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab15102 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/100.
IHC-P (3)
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
ICC/IF
1/100.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • Involvement in disease

    Note=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Target information above from: UniProt accession O15551 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1365 Human
    • Entrez Gene: 12739 Mouse
    • Entrez Gene: 65130 Rat
    • Omim: 602910 Human
    • SwissProt: O15551 Human
    • SwissProt: Q9Z0G9 Mouse
    • SwissProt: Q63400 Rat
    • Unigene: 647023 Human
    • Unigene: 158662 Mouse
    • Unigene: 4513 Rat
    see all
  • Alternative names

    • C7orf1 antibody
    • Claudin-3 antibody
    • Claudin3 antibody
    • CLD3_HUMAN antibody
    • CLDN 3 antibody
    • Cldn3 antibody
    • Clostridium perfringens enterotoxin receptor 2 antibody
    • CPE R2 antibody
    • CPE receptor 2 antibody
    • CPE-R 2 antibody
    • CPE-receptor 2 antibody
    • CPETR 2 antibody
    • CPETR2 antibody
    • HRVP 1 antibody
    • HRVP1 antibody
    • Rat ventral prostate 1 like protein antibody
    • Rat ventral prostate.1 protein homolog antibody
    • RVP1 antibody
    • Ventral prostate.1 like protein antibody
    • Ventral prostate.1 protein homolog antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)
    Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)
    ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)
    ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (51)

Publishing research using ab15102? Please let us know so that we can cite the reference in this datasheet.

ab15102 has been referenced in 51 publications.

  • Li E  et al. Mechanisms of deoxynivalenol-induced endocytosis and degradation of tight junction proteins in jejunal IPEC-J2 cells involve selective activation of the MAPK pathways. Arch Toxicol 95:2065-2079 (2021). PubMed: 33847777
  • Pang L  et al. STAT3 Signalling via the IL-6ST/gp130 Cytokine Receptor Promotes Epithelial Integrity and Intestinal Barrier Function during DSS-Induced Colitis. Biomedicines 9:N/A (2021). PubMed: 33673239
  • Schilpp C  et al. TGF-ß1 increases permeability of ciliated airway epithelia via redistribution of claudin 3 from tight junction into cell nuclei. Pflugers Arch 473:287-311 (2021). PubMed: 33386991
  • Romero-Cordoba SL  et al. Comprehensive omic characterization of breast cancer in Mexican-Hispanic women. Nat Commun 12:2245 (2021). PubMed: 33854067
  • Wang W  et al. NEO100 enables brain delivery of blood?brain barrier impermeable therapeutics. Neuro Oncol 23:63-75 (2021). PubMed: 32877532
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Claudin 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer (pH 6,0)
Permeabilization
Yes - Triton X 100 (0,5 %)
Specification
Jejunum
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 100µg/mL · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 22 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Claudin 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mature Porcine Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Mature Porcine Jejunum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Alex Pasternak

Verified customer

Submitted Aug 26 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Claudin 3 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Liver)
Specification
Liver
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH 9.0
Permeabilization
Yes - PBS-Triton 0,1% 10min
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Daniel Alpern

Verified customer

Submitted Aug 06 2012

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