Anti-Claudin 3 antibody (ab15102)

Thank you for contacting us. We have tested this antibody only in ICC/IF, WB and IHC-Phowevernot inflow cytometry. This antibody can be used in Flow Cytometry however due to lack of tested data we are unable to confirm it. Therefore, I can offer a discount off a future purchase if you buy ab15102 now, test it inFlow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free (PRIMARY ANTIBODY) If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab15102 in Flow Cyto. I will then send a discount code. This code must be issued before purchasing ab15102 so please wait for my reply before ordering. 2. Purchase ab15102 either by phone, fax, or online (www.abcam.com). 3. Test it in Flow Cyto. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY) ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15102 turns out to be unsuitable for Flow Cyto, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. PS: We recommend permeabilizing the cell when using this antibody in Flow cytometry.

Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab15102. This antibody hasn't previously been tested for application in Western blotting, so we don't know if it detects claudin 3 under these conditions. The antibody was characterized for application in IHC-formalin-fixed paraffin-embedded sections with small intestine cells. I would suggest that you run a positive control with the antibody; I found in SwissProt that claudin 3 is expressed at high levels in liver and lung and, at lower levels, in kidney and testis so you may want to try a liver or lung lysate. The predicted MW of claudin 3 is approximately 23 kDa and you're seeing a band at approximately 30 kDa, which is pretty close. The protein sample may have multiple modified forms (such as glycosylation, etc) so check the literature to see and check if there are any reports of variants. Also, I think that you can reduce the amount of lysate that you are loading t0 30 ug, which should be sufficient. I do suggest that you submit an Abreview. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. I hope this helps. Please contact us again if you have any additional questions.