Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Chicken, Human, Pig
- Isotype: IgG
Product nameAnti-Claudin 3 antibody
See all Claudin 3 primary antibodies
DescriptionRabbit polyclonal to Claudin 3
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Chicken, Human, Pig
Synthetic peptide within Mouse Claudin 3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Z0G9
- Tested with small intestine cells.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact firstname.lastname@example.org.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. PubMed: 18192323|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence analysis of pig primary porcine brain capillary endothelial cells labeling Claudin 3 with ab15102 (undiluted). Cells were fixed with Acetone/Methanol (3:7) and blocked with 10% BSA for 30 minutes at 22°C. A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate secondary antibody was used at 1/200. DAPI nuclear staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Claudin 3 with ab15102 at 1/300 dilution. Slides were rehydrated and then boiled for antigen retrieval in TE pH 9 buffer for 15min in standard microwave oven at 650W. After 30min blocking in PBS-Triton 0,1% / BSA 5% slides were incubated overnight with Anti-Claudin 3 antibody (ab15102) at 1/300 dilution at 4°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/200 dilution for 1h. Nuclei were stained with Hoechst.
Immunohistochemical analysis of PFA-fixed, paraffin embedded BALB/c murine ear tissue sections.
Claudin 3 was stained, using ab15102 at 1/10 dilution, in the inner ear at the organ of Corti, Reissner’s membrane, periosteal lining of otic capsule, and the apical surface of the marginal cells of stria vascularis (upper panel) and in the middle ear intercellular junctions along lateral and basal cell membranes in the mucosal epithelium (lower panel). An AlexaFluor®488-conjugated IgG (1/50) was used as the secondary antibody.
ab15102 has been referenced in 30 publications.
- Li Y et al. Protective effect of emodin on intestinal epithelial tight junction barrier integrity in rats with sepsis induced by cecal ligation and puncture. Exp Ther Med 19:3521-3530 (2020). PubMed: 32346413
- Lei Z et al. Dihydroartemisinin ameliorates dextran sulfate sodium induced inflammatory bowel diseases in mice. Bioorg Chem 100:103915 (2020). PubMed: 32450383
- Handa T et al. Carboxypeptidase A4 accumulation is associated with an aggressive phenotype and poor prognosis in triple-negative breast cancer. Int J Oncol 54:833-844 (2019). PubMed: 30628666
- Skálová H et al. Impact of chemotherapy on the expression of claudins and cadherins in invasive breast cancer. Exp Ther Med 18:3014-3024 (2019). PubMed: 31572543
- Chen Q et al. Effects of methionine partially replaced by methionyl-methionine dipeptide on intestinal function in methionine-deficient pregnant mice. J Anim Physiol Anim Nutr (Berl) 103:1610-1618 (2019). PubMed: 31106911