Anti-Claudin 3 antibody (ab15102)
- Datasheet
- References (21)
- Protocols
Overview
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Product nameAnti-Claudin 3 antibody
See all Claudin 3 primary antibodies -
DescriptionRabbit polyclonal to Claudin 3
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Chicken, Human, Pig
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Immunogen
Synthetic peptide within Mouse Claudin 3 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: Q9Z0G9 -
Positive control
- Tested with small intestine cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab15102 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100. | |
| WB | Use at an assay dependent concentration. PubMed: 18192323 | |
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
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Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
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Sequence similaritiesBelongs to the claudin family.
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Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1365 Human
- Entrez Gene: 12739 Mouse
- Entrez Gene: 65130 Rat
- Omim: 602910 Human
- SwissProt: O15551 Human
- SwissProt: Q9Z0G9 Mouse
- SwissProt: Q63400 Rat
- Unigene: 647023 Human
see all -
Alternative names
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)ICC/IF image of ab15102 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15102, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)ab15102 (2µg/ml) staining Claudin 3 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong membrane staining of the mucosal epithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Immunocytochemistry/ Immunofluorescence - Anti-Claudin 3 antibody (ab15102)This image is courtesy of an abreview submitted by Julia Stab, Fraunhofer IBMT.Immunocytochemistry/ Immunofluorescence analysis of pig primary porcine brain capillary endothelial cells labeling Claudin 3 with ab15102 (undiluted). Cells were fixed with Acetone/Methanol (3:7) and blocked with 10% BSA for 30 minutes at 22°C. A Goat anti-Rabbit IgG (H+L) Alexa Fluor® 488 conjugate secondary antibody was used at 1/200. DAPI nuclear staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)This image is courtesy of an abreview submitted by Daniel Alpern.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling Claudin 3 with ab15102 at 1/300 dilution. Slides were rehydrated and then boiled for antigen retrieval in TE pH 9 buffer for 15min in standard microwave oven at 650W. After 30min blocking in PBS-Triton 0,1% / BSA 5% slides were incubated overnight with Anti-Claudin 3 antibody (ab15102) at 1/300 dilution at 4°C. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/200 dilution for 1h. Nuclei were stained with Hoechst.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 3 antibody (ab15102)Image from Morris LM et al., PLoS One. 2012;7(6):e39004. Epub 2012 Jun 15. Fig 2.; doi:10.1371/journal.pone.0039004; June 15, 2012, PLoS ONE 7(6): e39004.Immunohistochemical analysis of PFA-fixed, paraffin embedded BALB/c murine ear tissue sections.
Claudin 3 was stained, using ab15102 at 1/10 dilution, in the inner ear at the organ of Corti, Reissner’s membrane, periosteal lining of otic capsule, and the apical surface of the marginal cells of stria vascularis (upper panel) and in the middle ear intercellular junctions along lateral and basal cell membranes in the mucosal epithelium (lower panel). An AlexaFluor®488-conjugated IgG (1/50) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Tokuhara Y et al. Nuclear expression of claudin-3 in human colorectal adenocarcinoma cell lines and tissues. Oncol Lett 15:99-108 (2018). Read more (PubMed: 29285188) »
- Zeniya S et al. Angubindin-1 opens the blood-brain barrier in vivo for delivery of antisense oligonucleotide to the central nervous system. J Control Release 283:126-134 (2018). ICC/IF ; Rat . Read more (PubMed: 29753959) »
