Key features and details
- Rabbit polyclonal to Claudin 3
- Suitable for: ELISA, WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Claudin 3 antibody
See all Claudin 3 primary antibodies
DescriptionRabbit polyclonal to Claudin 3
Specificityab52231 detects endogenous levels of total Claudin 3 protein.
Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide derived from within amino acids 181 and 220 of Human Claudin-3
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab52231 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|ICC/IF||Use a concentration of 5 µg/ml.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
All lanes : Anti-Claudin 3 antibody (ab52231) at 1/500 dilution
Lane 1 : Extracts from HuvEc cells, untreated.
Lane 2 : Extracts from HuvEc cells, treated with the immunizing peptide.
Predicted band size: 23 kDa
Observed band size: 23 kDa
IHC image of ab52231 staining in normal human colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52231, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab52231 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52231, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Claudin 3 antibody (ab52231)
Lane 1 : Human urothelial cells (differentiated)
Lane 2 : Human urothelial cells (non-differentiated)
Lysates/proteins at 15 µg per lane.
All lanes : IRDye® 800-conjugated Goat anti-rabbit IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 2 minutes
Blocked with 0.1% Milk for 1 hour at 22°C
ab52231 has been referenced in 4 publications.
- Vancamelbeke M et al. Butyrate does not protect against inflammation-induced loss of epithelial barrier function and cytokine production in primary cell monolayers from patients with ulcerative colitis. J Crohns Colitis N/A:N/A (2019). PubMed: 30919886
- Miranda J et al. Syncytiotrophoblast of Placentae from Women with Zika Virus Infection Has Altered Tight Junction Protein Expression and Increased Paracellular Permeability. Cells 8:N/A (2019). PubMed: 31569528
- Han CL et al. An Informatics-assisted Label-free Approach for Personalized Tissue Membrane Proteomics: Case Study on Colorectal Cancer. Mol Cell Proteomics 10:M110.003087 (2011). WB ; Human . PubMed: 21209152
- Ye P et al. CD24 regulated gene expression and distribution of tight junction proteins is associated with altered barrier function in oral epithelial monolayers. BMC Cell Biol 10:2 (2009). PubMed: 19138432