Key features and details
- Rabbit polyclonal to Claudin 3 (phospho Y219)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Claudin 3 (phospho Y219) antibody
See all Claudin 3 primary antibodies
DescriptionRabbit polyclonal to Claudin 3 (phospho Y219)
SpecificityThis antibody detects endogenous levels of Claudin 3 only when phosphorylated at tyrosine 219.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Synthesized phosphopeptide derived from human Claudin 3 around the phosphorylation site of tyrosine 219.
- Extracts form COLO cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab78345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 23 kDa).|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
Involvement in diseaseNote=CLDN3 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- C7orf1 antibody
- Claudin-3 antibody
- Claudin3 antibody
All lanes : Anti-Claudin 3 (phospho Y219) antibody (ab78345) at 1/500 dilution
Lane 1 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins)
Lane 2 : Extracts from COLO cells treated with EGF (200ng/ml, 30mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 23 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
ab78345 has not yet been referenced specifically in any publications.