Product nameAnti-Claudin 5 antibody
See all Claudin 5 primary antibodies
DescriptionRabbit polyclonal to Claudin 5
Tested applicationsSuitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Mouse Claudin 5 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O00501
- IHC-P: Mouse and human lung tissue. ICC/IF: MCF7 and HBMEC cells. WB: Mouse lung tissue lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab15106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22547149|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
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Anti-Claudin 5 antibody (ab15106) at 1/1000 dilution + Mouse lung tissue lysate at 30 µg
HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution
Developed using the ECL technique.
Performed under non-reducing conditions.
Predicted band size: 24 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa (possible non-specific binding)
Exposure time: 1 minute
Blocked with 5% milk for 1 hour at 25°C.
Incubated for 15 hours at 4°C with the primary antibody.
ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
ICC/IF image of ab15106 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).
ab15106 staining Claudin 5 in the Human HBMEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.
This product has been referenced in:
- Roberts JM et al. Bilateral carotid artery stenosis causes unexpected early changes in brain extracellular matrix and blood-brain barrier integrity in mice. PLoS One 13:e0195765 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29649307) »
- Chelakkot C et al. Akkermansia muciniphila-derived extracellular vesicles influence gut permeability through the regulation of tight junctions. Exp Mol Med 50:e450 (2018). IHC-P ; Mouse . Read more (PubMed: 29472701) »