Overview

  • Product name
  • Description
    Rabbit polyclonal to Claudin 5
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Mouse Claudin 5 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O00501

  • Positive control
    • IHC-P: Mouse and human lung tissue. ICC/IF: MCF7 and HBMEC cells. WB: Mouse lung tissue lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 22547149
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
IHC-P 1/50.

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Images

  • ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab15106 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15106, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).

  • ab15106 staining Claudin 5 in the Human HBMEC cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 1% Triton in PBS and blocked with 10% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-Claudin 5 antibody (ab15106) at 1/1000 dilution + Mouse lung tissue lysate at 30 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under non-reducing conditions.

    Predicted band size: 24 kDa
    Observed band size: 22 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 35 kDa (possible non-specific binding)


    Exposure time: 1 minute


    Blocked with 5% milk for 1 hour at 25°C.

    Incubated for 15 hours at 4°C with the primary antibody.

    See Abreview

References

This product has been referenced in:
  • Roberts JM  et al. Bilateral carotid artery stenosis causes unexpected early changes in brain extracellular matrix and blood-brain barrier integrity in mice. PLoS One 13:e0195765 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29649307) »
  • Chelakkot C  et al. Akkermansia muciniphila-derived extracellular vesicles influence gut permeability through the regulation of tight junctions. Exp Mol Med 50:e450 (2018). IHC-P ; Mouse . Read more (PubMed: 29472701) »

See all 20 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
40 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 09 2018

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary endothelial cells)
Permeabilization
Yes - 0.05% triton in PBS
Specification
primary endothelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Sheng-fu Huang

Verified customer

Submitted Jul 24 2017

Application
Western blot
Sample
Mouse Cell lysate - whole cell (neuroblastoma)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2017

Application
Western blot
Sample
Human Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
African green monkey Cell lysate - whole cell (Kidney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 05 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (Kidney)
Permeabilization
Yes - 0.1% Troton X-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 24 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% Troton X-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 19 2017

Application
Immunocytochemistry
Sample
Mouse Cell (bEnd.3)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
bEnd.3
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 25 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HBMEC)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
HBMEC
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 25 2015

1-10 of 16 Abreviews or Q&A

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