Anti-Claudin 5 antibody (ab15106)
Key features and details
- Rabbit polyclonal to Claudin 5
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Claudin 5 antibody
See all Claudin 5 primary antibodies -
Description
Rabbit polyclonal to Claudin 5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human lung tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/50.
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Notes |
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IHC-P
1/50. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7122 Human
- Entrez Gene: 12741 Mouse
- Omim: 602101 Human
- SwissProt: O00501 Human
- SwissProt: O54942 Mouse
- Unigene: 505337 Human
- Unigene: 22768 Mouse
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Alternative names
- Androgen withdrawal and apoptosis induced protein RVP1 like antibody
- AWAL antibody
- BEC 1 antibody
see all
Images
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ab15106 staining Claudin 5 in Mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1.5% serum for 1 hour at 37°C; antigen retrieval was by heat mediation in a acetic acid. Samples were incubated with primary antibody (1/200 in blocking buffer) for 18 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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ab15106 staining Claudin 5 in human lung by Immunohistochemistry (FFPE-sections).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (116)
ab15106 has been referenced in 116 publications.
- Lan G et al. Astrocytic VEGFA: An essential mediator in blood-brain-barrier disruption in Parkinson's disease. Glia 70:337-353 (2022). PubMed: 34713920
- Hajal C et al. Engineered human blood-brain barrier microfluidic model for vascular permeability analyses. Nat Protoc 17:95-128 (2022). PubMed: 34997242
- Harati R et al. miR-27a-3p regulates expression of intercellular junctions at the brain endothelium and controls the endothelial barrier permeability. PLoS One 17:e0262152 (2022). PubMed: 35025943
- Vdovenko D et al. Microvesicles released from activated CD4+ T cells alter microvascular endothelial cell function. Eur J Clin Invest 52:e13769 (2022). PubMed: 35316536
- Tang CF et al. Short-Chain Fatty Acids Ameliorate Depressive-like Behaviors of High Fructose-Fed Mice by Rescuing Hippocampal Neurogenesis Decline and Blood-Brain Barrier Damage. Nutrients 14:N/A (2022). PubMed: 35565849