Overview

  • Product name

    Anti-Claudin 5 antibody [EPR7583]
    See all Claudin 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7583] to Claudin 5
  • Host species

    Rabbit
  • Specificity

    ab131259 failed to detect target band in mouse brain tissue.

    Mouse species is recommended based on WB result. This antibody is unsuitable to be used in IHC on mouse tissues.

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Claudin 5 aa 200 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: O00501

  • Positive control

    • Human fetal brain, lung squamous cell cancer and small cell lung cancer, and Mouse lung lysates; Human kidney and lung tissues.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7583
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab131259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

ab131259 failed to detect target band in mouse brain tissue.

IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Images

  • All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution

    Lane 1 : Mouse lung tissue lysates with 5% NFDM/TBST
    Lane 2 : Rat lung tissue lysates with 5% NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 23 kDa
    Observed band size: 20 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    We didn’t recommend rat for that ab131259 failed to detect target band in rat lung in which Claudin 5 is highly expressed.

  • Immunohistochemical staining of paraffin embedded human colon with purified ab131259 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • All lanes : Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/1000 dilution (unpurified)

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human lung squamous cell cancer lysate
    Lane 3 : Human small cell lung cancer lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

    Predicted band size: 23 kDa

  • Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/5000 dilution (purified) + Human small cell lung cancer at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 23 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Anti-Claudin 5 antibody [EPR7583] (ab131259) at 1/20000 dilution (purified) + Human lung squamous cell cancer lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 23 kDa
    Observed band size: 20 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

  • Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

  • Unpurified ab131259 showing positive staining in Normal tonsil tissue.

  • Unpurified ab131259 showing positive staining in Normal placenta tissue.

  • Unpurified ab131259 showing positive staining in Normal uterus tissue.

  • Unpurified ab131259 showing positive staining in Normal colon tissue.

References

This product has been referenced in:

  • Peng H  et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . Read more (PubMed: 29636872) »
  • Lambrechts D  et al. Phenotype molding of stromal cells in the lung tumor microenvironment. Nat Med 24:1277-1289 (2018). Read more (PubMed: 29988129) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision FLEX Target Retrieval Solution
Specification
Brain
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 06 2018

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton X-100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 09 2014

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