Recombinant
RabMAb

Recombinant Anti-Claudin 5 antibody [EPR7583] - BSA and Azide free (ab236066)

Overview

  • Product name

    Anti-Claudin 5 antibody [EPR7583] - BSA and Azide free
    See all Claudin 5 primary antibodies
  • Description

    Rabbit monoclonal [EPR7583] to Claudin 5 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    ab236066 failed to detect target band in mouse brain tissue.

  • Tested applications

    Suitable for: IHC-Fr, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human Claudin 5 aa 200 to the C-terminus (intracellular). The exact sequence is proprietary.
    Database link: O00501

  • Positive control

    • IHC-P: Human colon, tonsil, lung, kidney, placenta and uterus tissue.
  • General notes

    ab236066 is the carrier-free version of ab131259 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236066 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7583
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).

ab236066 failed to detect target band in mouse brain tissue.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
    • AWAL antibody
    • BEC 1 antibody
    • BEC1 antibody
    • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
    • Claudin-5 antibody
    • Claudin5 antibody
    • CLD5_HUMAN antibody
    • CLDN 5 antibody
    • Cldn5 antibody
    • CPETR L1 antibody
    • CPETRL 1 antibody
    • CPETRL1 antibody
    • TMDVCF antibody
    • TMVCF antibody
    • Transmembrane protein deleted in VCFS antibody
    • Transmembrane protein deleted in velocardiofacial syndrome antibody
    see all

Images

  • Imaging Mass Cytometry™ (IMC™) image of human tonsil tissue stained with anti-Claudin 5 antibody [EPR7583]. ab236066 (carrier-free antibody, purified) was metal-conjugated using a Maxpar® Antibody Labeling Kit from Fluidigm. Immunostaining was performed according to Fluidigm’s protocols. Briefly, slides were subject to deparaffinization and heat-induced epitope retrieval, followed by overnight incubation at 4°C with an antibody cocktail containing metal-tagged antibodies in blocking buffer. Slides were subsequently washed with 0.2% Triton-X and 1x PBS, counterstained with Cell-ID™ Intercalator-Ir diluted at 1/400 in 1x PBS for 30 min at room temperature, rinsed for 5 min with distilled H2O, and air-dried prior to IMC™ acquisition. IMC™ acquisition was performed using the Fluidigm Hyperion™ Imaging System.

    Imaging Mass Cytometry™, IMC™, Cell-ID™, Hyperion™ and Maxpar® are trademarks of Fluidigm Canada

  • Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical analysis of paraffin embedded Human lung tissue labelling Claudin 5 with unpurified ab131259 antibody at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Unpurified ab131259 showing positive staining in Normal tonsil tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Unpurified ab131259 showing positive staining in Normal placenta tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Unpurified ab131259 showing positive staining in Normal uterus tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Unpurified ab131259 showing positive staining in Normal colon tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemical staining of paraffin embedded human colon with purified ab131259 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131259).

References

ab236066 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab236066.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up