Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
- Suitable for: WB, IP
- Reacts with: Human
Product nameAnti-Claudin 5 (phospho Y217) antibody [EPR10810]
See all Claudin 5 primary antibodies
DescriptionRabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Predicted to work with: RatDoes not react with: Mouse
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Claudin 5 aa 200 to the C-terminus (phospho Y217). The exact sequence is proprietary.
Database link: O00501
- MCF7 cell lysate, treated with pervanadate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/50000 - 1/200000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).|
|IP||1/10 - 1/100.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- Androgen withdrawal and apoptosis induced protein RVP1 like antibody
- AWAL antibody
- BEC 1 antibody
All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution
Lane 1 : MCF7 cell lysate, untreated
Lane 2 : MCF7 cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa
Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab172968 has been referenced in 1 publication.
- Peng H et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . PubMed: 29636872