Overview

  • Product name
    Anti-Claudin 5 (phospho Y217) antibody [EPR10810]
    See all Claudin 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Claudin 5 aa 200 to the C-terminus (phospho Y217). The exact sequence is proprietary.
    Database link: O00501

  • Positive control
    • MCF7 cell lysate, treated with pervanadate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10810
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50000 - 1/200000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Plays a major role in tight junction-specific obliteration of the intercellular space.
    • Sequence similarities
      Belongs to the claudin family.
    • Cellular localization
      Cell junction > tight junction. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Androgen withdrawal and apoptosis induced protein RVP1 like antibody
      • AWAL antibody
      • BEC 1 antibody
      • BEC1 antibody
      • Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody
      • Claudin-5 antibody
      • Claudin5 antibody
      • CLD5_HUMAN antibody
      • CLDN 5 antibody
      • Cldn5 antibody
      • CPETR L1 antibody
      • CPETRL 1 antibody
      • CPETRL1 antibody
      • TMDVCF antibody
      • TMVCF antibody
      • Transmembrane protein deleted in VCFS antibody
      • Transmembrane protein deleted in velocardiofacial syndrome antibody
      see all

    Images

    • All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution

      Lane 1 : MCF7 cell lysate, untreated
      Lane 2 : MCF7 cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 23 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    This product has been referenced in:
    • Peng H  et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . Read more (PubMed: 29636872) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab172968.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up