Recombinant Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217)
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Claudin 5 (phospho Y217) antibody [EPR10810]
See all Claudin 5 primary antibodies -
Description
Rabbit monoclonal [EPR10810] to Claudin 5 (phospho Y217) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7 cell lysate, treated with pervanadate; A431 cells treated with 50mM Pervanadate for 5 minutes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10810 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/50000 - 1/200000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
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IP |
1/10 - 1/100.
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Notes |
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WB
1/50000 - 1/200000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
IP
1/10 - 1/100. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7122 Human
- Entrez Gene: 65131 Rat
- Omim: 602101 Human
- SwissProt: O00501 Human
- SwissProt: Q9JKD6 Rat
- Unigene: 505337 Human
- Unigene: 17420 Rat
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Alternative names
- Androgen withdrawal and apoptosis induced protein RVP1 like antibody
- AWAL antibody
- BEC 1 antibody
see all
Images
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All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/1000 dilution
Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate
Lane 3 : A431 (Human epidermoid carcinoma epithelial cell) treated with 50mM Pervanadate for 5 minutes whole cell lysate, the membrane was treated with alkaline phosphatase for 1 hour
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 23 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as a GAPDH loading control.
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All lanes : Anti-Claudin 5 (phospho Y217) antibody [EPR10810] (ab172968) at 1/50000 dilution
Lane 1 : MCF7 cell lysate, untreated
Lane 2 : MCF7 cell lysate, treated with pervanadate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
Western blot analysis on immunoprecipitation pellet from (1) MCF7 cells treated with pervanadate lysate or (2) 1X PBS (negative control) using ab172968 Phospho (pY217) at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab172968 has been referenced in 5 publications.
- Huang LY et al. Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model. Front Cell Neurosci 16:841544 (2022). PubMed: 35308117
- Zhu N et al. Claudin-5 relieves cognitive decline in Alzheimer's disease mice through suppression of inhibitory GABAergic neurotransmission. Aging (Albany NY) 14:3554-3568 (2022). PubMed: 35471411
- Cai H et al. Momordica charantia Exosome-Like Nanoparticles Exert Neuroprotective Effects Against Ischemic Brain Injury via Inhibiting Matrix Metalloproteinase 9 and Activating the AKT/GSK3β Signaling Pathway. Front Pharmacol 13:908830 (2022). PubMed: 35814200
- Cao Q et al. miR-151-5p alleviates corneal allograft rejection by activating PI3K/AKT signaling pathway and balancing Th17/Treg after corneal transplantation via targeting IL-2Rɑ. Ann Transl Med 9:1410 (2021). PubMed: 34733962
- Peng H et al. Qihuang decoction promotes the recovery of intestinal immune barrier dysfunction after gastrectomy in rats. Am J Transl Res 10:827-836 (2018). WB ; Rat . PubMed: 29636872