Product nameAnti-Claudin 6 antibody
See all Claudin 6 primary antibodies
DescriptionRabbit polyclonal to Claudin 6
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide between amino acids 133-163 from the central region of Human Claudin 6 (NP_067018.2), conjugated to KLH.
- Mouse heart tissue lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human normal kidney.
Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab107059 is purified through a protein A column, followed by peptide affinity purification.
Our Abpromise guarantee covers the use of ab107059 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Predicted molecular weight: 23 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- Claudin-6 antibody
- Claudin6 antibody
- CLD6_HUMAN antibody
Anti-Claudin 6 antibody (ab107059) at 1/100 dilution + Mouse heart tissue lysate at 35 µg
Predicted band size: 23 kDa
ab107059 staining Claudin 6 in murine AGDC 5 chondocytic cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldhyde and permeabilized in 0.1% Triton X-100 prior to blocking in 10% FBS in PBS for 2 hours at room temperature. The primary antibody was diluted 1/50 and incubated with the sample for 12 hours at 4°C with overnight gyratory incubation. The secondary antibody was Alexa Fluor® 594-conjugated goat anti-Rabbit polyclonal, diluted 1/50.
Red staining is Claudin 6 and the blue is nuclear counterstaining with DAPI. Oil immersion microscopy was at 100X using an Olympus BX21 fluorescent microscope with an Infinity 3 camera.
All lanes : Anti-Claudin 6 antibody (ab107059) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from mouse frontal cortex.
Lane 2 : Whole tissue lysate prepared from mouse temporal cortex.
Lane 3 : Whole tissue lysate prepared from mouse occipital cortex.
Lane 4 : Whole tissue lysate prepared from mouse cerebellum.
Lane 5 : Whole tissue lysate prepared from mouse brainstem.
Lane 6 : Whole tissue lysate prepared from mouse muscle.
Lane 7 : Whole tissue lysate prepared from mouse liver.
Lane 8 : Whole tissue lysate prepared from mouse lung.
Lysates/proteins at 24 µg per lane.
All lanes : Goat anti-rabbit IgG conjugated to HRP at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 10 seconds
IHC image of Claudin 6 staining in Human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107059, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.