Overview

  • Product name

  • Description

    Rabbit polyclonal to Claudin 6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Claudin 6 aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab113301)

  • Positive control

    • This antibody gave a positive signal in the following lysates: A498 (Human Kidney Carcinoma) Whole Cell Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab99226 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 23 kDa).

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Claudin-6 antibody
    • Claudin6 antibody
    • CLD6_HUMAN antibody
    • CLDN 6 antibody
    • CLDN6 antibody
    • OTTHUMP00000159248 antibody
    • Skullin 2 antibody
    • Skullin antibody
    • UNQ757/PRO1488 antibody
    see all

Images

  • Anti-Claudin 6 antibody (ab99226) at 1 µg/ml + A498 (Human Kidney Carcinoma) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 24 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 47 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

ab99226 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Human CLDN6 overexpression)
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Loading amount
20 µg
Specification
Human CLDN6 overexpression
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 02 2016

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