Product nameAnti-Claudin 7 / CLDN-7 antibody
See all Claudin 7 / CLDN-7 primary antibodies
DescriptionRabbit polyclonal to Claudin 7 / CLDN-7
Tested applicationsSuitable for: ICC, IHC-P, IHC-Fr, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Claudin 7/ CLDN-7 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: O95471
- Human colon carcinoma.
Previously labelled as Claudin 7.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 0.0268% PBS, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAb27487 is affinity purified.
Our Abpromise guarantee covers the use of ab27487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 21525338|
|WB||1/100. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Tissue specificityExpressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
- Information by UniProt
- CEPTR L2 antibody
- CEPTRL 2 antibody
- CEPTRL2 antibody
Ab27487, at a dilution of 1/200, staining Claudin 7 / CLDN-7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.
ab27487 staining tissue sections of rat liver after oval cell activation. The sample was PFA fixed, permeabilized in 0,1% Triton X100 for 10 minutes and blocked with 2% serum, 2%BSA, 0.05% Tween 20 in PBS for 1 hour at room temperature. The primary antibody was diluted 1/100 and incubated for 12 hours at 4°C. A HRP conjugated donkey anti-rabbit was used as the secondary.
ab27487 staining rat oval cells by ICC. Cells were fixed in ice cold methanol for 15 minutes and blocked in 3% serum for 1 hour at 25°C prior to incubation with ab27487 (diluted 1/50 in 3% Goat serum, 2% BSA, 0.01% Tween20) for 16 hours at 4°C. The secondary antibody was a Cy3® conjugated donkey anti-rabbit, diluted 1/100.
All lanes : Anti-Claudin 7 / CLDN-7 antibody (ab27487) at 1/100 dilution
Lane 1 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - Insoluble fraction (membrane)
Lane 2 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - soluble fraction (cytoplasm)
Lysates/proteins at 22 µg per lane.
All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Blocking Step: 5% BSA with Tris Base Salt buffer (TBS, pH7.5) and Tween (0.05%) for 1 hour at 22°C
This product has been referenced in:
- Tokuhara Y et al. Nuclear expression of claudin-3 in human colorectal adenocarcinoma cell lines and tissues. Oncol Lett 15:99-108 (2018). Read more (PubMed: 29285188) »
- Aiello NM et al. EMT Subtype Influences Epithelial Plasticity and Mode of Cell Migration. Dev Cell 45:681-695.e4 (2018). Read more (PubMed: 29920274) »