Overview

  • Product name
    Anti-Claudin 7 / CLDN-7 antibody
    See all Claudin 7 / CLDN-7 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 7 / CLDN-7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC, IHC-P, IHC-Fr, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Claudin 7/ CLDN-7 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: O95471

  • Positive control
    • Human colon carcinoma.
  • General notes

    Previously labelled as Claudin 7. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Ab27487 is affinity purified.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/50.
IHC-P 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. In particular, boil tissue sections in 10mM citrate buffer pH 6.0 for 10 min then cool at room temperature for 20 min.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 21525338
WB 1/100. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity
    Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
  • Sequence similarities
    Belongs to the claudin family.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt
  • Database links
  • Alternative names
    • CEPTR L2 antibody
    • CEPTRL 2 antibody
    • CEPTRL2 antibody
    • Claudin 1 antibody
    • Claudin 7 antibody
    • Claudin 9 antibody
    • Claudin-7 antibody
    • Claudin1 antibody
    • Claudin7 antibody
    • Claudin9 antibody
    • CLD7_HUMAN antibody
    • CLDN 7 antibody
    • CLDN-7 antibody
    • CLDN7 antibody
    • Clostridium perfringens enterotoxin receptor like 2 antibody
    • CPETR L2 antibody
    • CPETRL 2 antibody
    • CPETRL2 antibody
    • Hs.84359 antibody
    see all

Images

  • Ab27487, at a dilution of 1/200, staining Claudin 7 / CLDN-7 in formalin fixed paraffin embedded human colon carcinoma tissue section by Immunohistochemistry.

  • ab27487 staining tissue sections of rat liver after oval cell activation.  The sample was PFA fixed, permeabilized in 0,1% Triton X100 for 10 minutes and blocked with 2% serum, 2%BSA, 0.05% Tween 20 in PBS for 1 hour at room temperature.  The primary antibody was diluted 1/100 and incubated for 12 hours at 4°C.  A HRP conjugated donkey anti-rabbit was used as the secondary.

    See Abreview

  • ab27487 staining rat oval cells by ICC.  Cells were fixed in ice cold methanol for 15 minutes and blocked in 3% serum for 1 hour at 25°C prior to incubation with ab27487 (diluted 1/50 in 3% Goat serum, 2% BSA, 0.01% Tween20) for 16 hours at 4°C.  The secondary antibody was a Cy3® conjugated donkey anti-rabbit, diluted 1/100.

    See Abreview

  • All lanes : Anti-Claudin 7 / CLDN-7 antibody (ab27487) at 1/100 dilution

    Lane 1 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - Insoluble fraction (membrane)
    Lane 2 : Whole cell lysate of human Colon Adenocarcinoma epithelial cells C2BBe1 - soluble fraction (cytoplasm)

    Lysates/proteins at 22 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 22 kDa
    Observed band size: 22 kDa
    Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 3 minutes


    Blocking Step: 5% BSA with Tris Base Salt buffer (TBS, pH7.5) and Tween (0.05%) for 1 hour at 22°C

    See Abreview

References

This product has been referenced in:
See all 17 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Application
Western blot
Sample
Pig Tissue lysate - whole (Intestine)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Specification
Intestine
Blocking step
Odyssey blocking as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 11 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Mature Porcine Jejunum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 6.0
Permeabilization
No
Specification
Mature Porcine Jejunum
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Alex Pasternak

Verified customer

Submitted Aug 26 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (15%)
Loading amount
30 µg
Specification
lung
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 08 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Small intestine
Blocking step
Dako serum free protein block as blocking agent as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 01 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 04 2014

Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Non-Denaturing (Native)
Loading amount
20 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (colon)
Permeabilization
Yes - boil tissue sections in 10mM citrate buffer pH 6.0
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted May 05 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Specification
liver cancer
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Colon Adenocarcinoma epithelial cells C2BBe1)
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris gels)
Loading amount
22 µg
Specification
Human Colon Adenocarcinoma epithelial cells C2BBe1
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted May 27 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Rat Cell (oval cells)
Permeabilization
No
Specification
oval cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
ice cold methanol 15 min

Abcam user community

Verified customer

Submitted Apr 08 2008

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up