Product nameAnti-Claudin 7/CLDN-7 antibody
See all Claudin 7/CLDN-7 primary antibodies
DescriptionRabbit polyclonal to Claudin 7/CLDN-7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Claudin 7/CLDN-7 aa 161-211. The exact sequence is proprietary.
Database link: O95471
- WB: MCF-7, LNCaP and HepG2 whole cell lysate. IP: MCF7 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab265583 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 22 kDa.|
|IP||Use at an assay dependent concentration.
20-40 µl/mg lysate
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Tissue specificityExpressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
- Information by UniProt
- CEPTR L2 antibody
- CEPTRL 2 antibody
- CEPTRL2 antibody
All lanes : Anti-Claudin 7/CLDN-7 antibody (ab265583) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : LNCap whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 22 kDa
Exposure time: 75 seconds
CLDN7 was immuoprecipitated from 1 mg MCF7 whole cell lysate using ab265583 at 40 µL per reaction. Western blot was performed on the immunoprecipitate using ab265583 at 1/1000 dilution.
Lane 1: ab265583 IP in MCF7 whole cell lysate.
Lane 2: Control IgG.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab265583 has not yet been referenced specifically in any publications.