RecombinantRabMAb

Recombinant Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Flow Cytometry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
  • Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18073] to Claudin 7/CLDN-7 - BSA and Azide free
  • Suitable for: Flow Cyt, ICC, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free
    See all Claudin 7/CLDN-7 primary antibodies

  • Description

    Rabbit monoclonal [EPR18073] to Claudin 7/CLDN-7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Claudin 7/CLDN-7 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: O95471

  • Positive control

    • IHC-P; Human renal oncocytoma and Human endometrial cancer tissue. ICC/IF; MCF7 and SW480 cells. FC; MCF7 cells.

  • General notes

    Ab214576 is the carrier-free version of ab207300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214576 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR18073

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab214576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.

  • Tissue specificity

    Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.

  • Sequence similarities

    Belongs to the claudin family.

  • Post-translational
    modifications

    Phosphorylated.

  • Cellular localization

    Cell membrane. Lateral cell membrane. Cell junction > tight junction. Co-localizes with EPCAM at the lateral cell membrane and tight junction.
  • Information by UniProt

  • Database links

    • Alternative names

      • CEPTR L2 antibody
      • CEPTRL 2 antibody
      • CEPTRL2 antibody
      • Claudin 1 antibody
      • Claudin 7 antibody
      • Claudin 9 antibody
      • Claudin-7 antibody
      • Claudin1 antibody
      • Claudin7 antibody
      • Claudin9 antibody
      • CLD7_HUMAN antibody
      • CLDN 7 antibody
      • CLDN-7 antibody
      • CLDN7 antibody
      • Clostridium perfringens enterotoxin receptor like 2 antibody
      • CPETR L2 antibody
      • CPETRL 2 antibody
      • CPETRL2 antibody
      • Hs.84359 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)

      This data was developed using ab207300, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human renal oncocytoma tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human renal oncocytoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      This data was developed using ab207300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human renal clear cell carcinoma tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on human renal clear cell carcinoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      This data was developed using ab207300, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrial cancer tissue labeling Claudin 7/CLDN-7 with ab207300 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining on human endometrial cancer tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051). Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      This data was developed using ab207300, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on MCF7 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
    • Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Immunocytochemistry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      This data was developed using ab207300, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on SW480 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
    • Flow Cytometry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Flow Cytometry - Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      This data was developed using ab207300, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SW480 (Human colorectal adenocarcinoma cell line) cells labeling Claudin 7/CLDN-7 with ab207300 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Membranous staining on SW480 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab207300 at 1/250 dilution followed byab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
    • Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)
      Anti-Claudin 7/CLDN-7 antibody [EPR18073] - BSA and Azide free (ab214576)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab214576? Please let us know so that we can cite the reference in this datasheet.

    ab214576 has not yet been referenced specifically in any publications.

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