Key features and details
- Rabbit polyclonal to Claudin 8
- Suitable for: IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Claudin 8 antibody
See all Claudin 8 primary antibodies
DescriptionRabbit polyclonal to Claudin 8
Tested applicationsSuitable for: IHC-P, WB, Flow Cytmore details
Species reactivityReacts with: Human
- Human colon tissue; 293 cells; 293 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab211439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/1000. Predicted molecular weight: 25 kDa.|
|Flow Cyt||1/10 - 1/50.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space.
Tissue specificityExpressed in the epididymis, mainly in the caput segment.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
- Information by UniProt
- Claudin 8 antibody
- Claudin-8 antibody
- CLD8_HUMAN antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Claudin 8 with ab211439 at 1/75 dilution.
Anti-Claudin 8 antibody (ab211439) at 1/1000 dilution + 293 cell lysate at 35 µg
Predicted band size: 25 kDa
Flow cytometric analysis of 293 cells labeling Claudin 8 with ab211439 at 1/10 dilution (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab211439 has been referenced in 1 publication.
- Liu B et al. MiR-361-5p inhibits cell proliferation and induces cell apoptosis in retinoblastoma by negatively regulating CLDN8. Childs Nerv Syst 35:1303-1311 (2019). PubMed: 31161266