Overview

  • Product name

  • Description

    Rabbit polyclonal to Claudin 8
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Claudin 8 aa 140-166 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Sequence:

    VANAIIRDFYNSIVNVAQKRELGEALY


    Database link: P56748

  • Positive control

    • Human colon tissue; 293 cells; 293 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab211439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/75. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 25 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity

    Expressed in the epididymis, mainly in the caput segment.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • Claudin 8 antibody
    • Claudin-8 antibody
    • CLD8_HUMAN antibody
    • Cldn8 antibody
    • Epididymis secretory protein Li 79 antibody
    • HEL S 79 antibody
    • OTTHUMP00000101915 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Claudin 8 with ab211439 at 1/75 dilution.

  • Anti-Claudin 8 antibody (ab211439) at 1/1000 dilution + 293 cell lysate at 35 µg

    Predicted band size: 25 kDa

  • Flow cytometric analysis of 293 cells labeling Claudin 8 with ab211439 at 1/10 dilution (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab211439 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab211439.
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