Overview

  • Product name
    Anti-Claudin 8 antibody - Azide free
    See all Claudin 8 primary antibodies
  • Description
    Rabbit polyclonal to Claudin 8 - Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human)

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 25 kDa.

Target

  • Function
    Plays a major role in tight junction-specific obliteration of the intercellular space.
  • Tissue specificity
    Expressed in the epididymis, mainly in the caput segment.
  • Sequence similarities
    Belongs to the claudin family.
  • Cellular localization
    Cell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • Claudin 8 antibody
    • Claudin-8 antibody
    • CLD8_HUMAN antibody
    • Cldn8 antibody
    • Epididymis secretory protein Li 79 antibody
    • HEL S 79 antibody
    • OTTHUMP00000101915 antibody
    see all

References

ab37469 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for getting back to me. Generally if an immunogen forms an alignment of 6 or more consecutive residues I would consider it possible that the antibody would react against the homolog of the species. However, if it is a polyclonal antisera this is not always the case as given its polyclonal nature it is a mixture of immunoglobulin molecules. I would be very interested to know the results of your sequence comparisons as I could not find an immunogen that carried significant sequence similarity to warrant the suggestion of the application of the antibody to fish. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. I have performed a sequence analysis of your fish sequence with all of immunogens used to raise our antibodies against Claudin. Unfortunately none had significant sequence similarity that would make me consider reactivity likely. I am sorry that I cannot be of more assistance on this occasion.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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