RecombinantRabMAb

Recombinant Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
  • Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12680(2)] to Claudin 8
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Claudin 8 antibody [EPR12680(2)]
    See all Claudin 8 primary antibodies

  • Description

    Rabbit monoclonal [EPR12680(2)] to Claudin 8

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Claudin 8 aa 200 to the C-terminus. The exact sequence is proprietary.
    Database link: P56748

  • Positive control

    • Human colon and Human colonic adenocarcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR12680(2)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183738 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space.

  • Tissue specificity

    Expressed in the epididymis, mainly in the caput segment.

  • Sequence similarities

    Belongs to the claudin family.

  • Cellular localization

    Cell junction > tight junction. Cell membrane. Localizes to tight junctions in all 3 segments of the epididymis, in the caput found in the lateral margins of principal cells, and in the corpus at the interface between basal and principal cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Claudin 8 antibody
    • Claudin-8 antibody
    • CLD8_HUMAN antibody
    • Cldn8 antibody
    • Epididymis secretory protein Li 79 antibody
    • HEL S 79 antibody
    • OTTHUMP00000101915 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)

    Immunohistochemical analysis of paraffin-embedded Human colonic adenocarcinoma tissue labeling Claudin 8 with ab183738 at 1/1000 dilution. Counter stain Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)
    Anti-Claudin 8 antibody [EPR12680(2)] (ab183738)

References (5)

Publishing research using ab183738? Please let us know so that we can cite the reference in this datasheet.

ab183738 has been referenced in 5 publications.

  • Zhou S  et al. Identification of claudin-1, -3, -7 and -8 as prognostic markers in human laryngeal carcinoma. Mol Med Rep 20:393-400 (2019). PubMed: 31115553
  • Jia H  et al. Identification of claudin-2, -6, -11 and -14 as prognostic markers in human breast carcinoma. Int J Clin Exp Pathol 12:2195-2204 (2019). PubMed: 31934042
  • Zhang X  et al. Differences in the expression profiles of claudin proteins in human nasopharyngeal carcinoma compared with non-neoplastic mucosa. Diagn Pathol 13:11 (2018). PubMed: 29402318
  • Yang L  et al. Differences in the expression profiles of claudin proteins in human gastric carcinoma compared with non-neoplastic mucosa. Mol Med Rep 18:1271-1278 (2018). PubMed: 29901188
  • Ashikari D  et al. CLDN8, an androgen-regulated gene, promotes prostate cancer cell proliferation and migration. Cancer Sci 108:1386-1393 (2017). Human . PubMed: 28474805

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