Recombinant Anti-Claudin18 antibody [34H14L15] (ab203563)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [34H14L15] to Claudin18
- Suitable for: ICC, ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Claudin18 antibody [34H14L15]
See all Claudin18 primary antibodies -
Description
Rabbit monoclonal [34H14L15] to Claudin18 -
Host species
Rabbit -
Tested applications
Suitable for: ICC, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Claudin18 aa 200 to the C-terminus.
Database link: P56856 -
Positive control
- WB: Mouse lung lysate. Rat heart lysate. A431 and A549 lysates. IHC-P: Human stomach and squamous lung carcimona tissues. ICC/IF: Caco-2 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
34H14L15 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC |
1/100 - 1/1000.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB |
Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 28 kDa.
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IHC-P | (1) |
Use a concentration of 2 - 4 µg/ml.
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Notes |
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ICC
1/100 - 1/1000. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 28 kDa. |
IHC-P
Use a concentration of 2 - 4 µg/ml. |
Target
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Function
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
Tissue specificity
Concentrated at the cell-cell borders of epithelial cells. -
Sequence similarities
Belongs to the claudin family. -
Cellular localization
Cell junction > tight junction. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 51208 Human
- Entrez Gene: 56492 Mouse
- Entrez Gene: 315953 Rat
- Omim: 609210 Human
- SwissProt: P56856 Human
- SwissProt: P56857 Mouse
- Unigene: 655324 Human
- Unigene: 386784 Mouse
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Alternative names
- Claudin 18 antibody
- Claudin-18 antibody
- CLD18_HUMAN antibody
see all
Images
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Formalin-fixed, paraffin-embedded human stomach tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : A431 (Human epidermoid carcinoma cell line) cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/5000 dilution
Predicted band size: 28 kDa4-12% Bis-Tris gel
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Immunofluorescent analysis of Caco-2 (Human colorectal adenocarcinoma cell line) cells (4% paraformaldehyde-fixed) labeling Claudin18 with ab203563 at 1/500 dilution followed with an Alexa Fluor® 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. Panel c is a merged image showing cell junction localization and panel d is a control without primary antibody.
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Formalin-fixed, paraffin-embedded human squamous lung carcinoma tissue stained for Claudin18 with ab203563 at 2 µg/ml in immunohistochemical analysis. DAB staining.
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All lanes : Anti-Claudin18 antibody [34H14L15] (ab203563) at 2 µg/ml
Lane 1 : Mouse lung lysate
Lane 2 : Mouse lung lysate with immunizing peptide
Predicted band size: 28 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab203563 has been referenced in 5 publications.
- Sapoznikov A et al. Characterization of Lung Injury following Abrin Pulmonary Intoxication in Mice: Comparison to Ricin Poisoning. Toxins (Basel) 14:N/A (2022). PubMed: 36136552
- Zhou J et al. The Degradation of Airway Epithelial Tight Junctions in Asthma Under High Airway Pressure Is Probably Mediated by Piezo-1. Front Physiol 12:637790 (2021). PubMed: 33868003
- Sapoznikov A et al. Early disruption of the alveolar-capillary barrier in a ricin-induced ARDS mouse model: neutrophil-dependent and -independent impairment of junction proteins. Am J Physiol Lung Cell Mol Physiol 316:L255-L268 (2019). PubMed: 30382767
- Xu JH et al. Berberine protects against diet-induced obesity through regulating metabolic endotoxemia and gut hormone levels. Mol Med Rep 15:2765-2787 (2017). PubMed: 28447763
- Jiang M et al. Transitional basal cells at the squamous-columnar junction generate Barrett's oesophagus. Nature 550:529-533 (2017). IHC-Fr . PubMed: 29019984