Recombinant Anti-ClC-2 antibody [EPR6492(2)] (ab154798)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6492(2)] to ClC-2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-ClC-2 antibody [EPR6492(2)]
See all ClC-2 primary antibodies -
Description
Rabbit monoclonal [EPR6492(2)] to ClC-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human ClC-2. The exact sequence is proprietary.
(Peptide available asab218163) -
Positive control
- HT29 and HeLa whole cell lysate (ab150035); mouse brain, mouse heart, mouse kidney, mouse spleen, rat kidney and rat spleen lysates; Human thyroid gland carcinoma tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6492(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse kidney normal tissue lysate - total protein (ab29305)
- HeLa whole cell lysate (ab29545)
- Mouse brain cerebellum tissue lysate - total protein (ab30173)
- Mouse heart normal tissue lysate - total protein (ab30291)
- HT-29 whole cell lysate (ab3952)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154798 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 99 kDa.
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IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 99 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. -
Tissue specificity
Ubiquitously expressed. Moderately expressed in aortic and coronary vascular smooth muscle cells and expressed at a low level in aortic endothelial cells. -
Involvement in disease
Defects in CLCN2 are associated with susceptibility to idiopathic generalized epilepsy type 11 (IGE11) [MIM:607628]. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Generalized seizures arise diffusely and simultaneously from both hemispheres of the brain.
Defects in CLCN2 are the cause of childhood absence epilepsy type 3 (ECA3) [MIM:607682]. ECA3 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3 Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop.
Defects in CLCN2 are associated with juvenile absence epilepsy type 2 (JAE2) [MIM:607628]. JAE is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset occurring around puberty, absence seizures, generalized tonic-clonic seizures (GTCS), GTCS on awakening and myoclonic seizures.
Defects in CLCN2 are associated with juvenile myoclonic epilepsy type 8 (EJM8) [MIM:607628]. A subtype of idiopathic generalized epilepsy. Patients have afebrile seizures only, with onset in adolescence (rather than in childhood) and myoclonic jerks which usually occur after awakening and are triggered by sleep deprivation and fatigue. -
Sequence similarities
Belongs to the chloride channel (TC 2.A.49) family. ClC-2/CLCN2 subfamily.
Contains 2 CBS domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 1181 Human
- Entrez Gene: 12724 Mouse
- Entrez Gene: 29232 Rat
- Omim: 600570 Human
- SwissProt: P51788 Human
- SwissProt: Q6IPA9 Human
- SwissProt: Q9R0A1 Mouse
- SwissProt: P35525 Rat
see all -
Alternative names
- Chloride Channel 2 antibody
- Chloride channel protein 2 antibody
- Chloride channel, voltage sensitive 2 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human thyroid gland carcinoma tissue labeling ClC-2 with ab154798 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-ClC-2 antibody [EPR6492(2)] (ab154798) at 1/1000 dilution
Lane 1 : HT29 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa -
All lanes : Anti-ClC-2 antibody [EPR6492(2)] (ab154798) at 1/1000 dilution
Lane 1 : mouse brain lysate
Lane 2 : mouse heart lysate
Lane 3 : mouse kidney lysate
Lane 4 : mouse spleen lysate
Lane 5 : rat kidney lysate
Lane 6 : rat spleen lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 99 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154798 has been referenced in 2 publications.
- Ding W et al. ClC-2 inhibition prevents macrophage foam cell formation by suppressing Nlrp3 inflammasome activation. Biosci Biotechnol Biochem 84:2096-2103 (2020). PubMed: 32657644
- Sun L et al. The CLC-2 Chloride Channel Modulates ECM Synthesis, Differentiation, and Migration of Human Conjunctival Fibroblasts via the PI3K/Akt Signaling Pathway. Int J Mol Sci 17:N/A (2016). WB ; Human . PubMed: 27294913