Key features and details
- Rabbit polyclonal to ClC-4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ClC-4 antibody
DescriptionRabbit polyclonal to ClC-4
Specificityab75008 detects endogenous levels of total ClC-4 protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human ClC-4 (internal sequence).
- WB: Extracts from MCF-7 cells ICC/IF: HUVEC cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab75008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 85 kDa.|
|ICC/IF||1/500 - 1/1000.|
FunctionProton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons.
Tissue specificityAbundant in skeletal muscle and also detectable in brain and heart.
Sequence similaritiesBelongs to the chloride channel (TC 2.A.49) family. ClC-4/CLCN4 subfamily.
Contains 2 CBS domains.
Cellular localizationEarly endosome membrane. Late endosome membrane.
- Information by UniProt
- Chloride channel 4 antibody
- Chloride channel protein 4 antibody
- Chloride channel, voltage sensitive 4 antibody
All lanes : Anti-ClC-4 antibody (ab75008) at 1/500 dilution
Lane 1 : extracts from MCF-7 cells
Lane 2 : extracts from MCF-7 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 85 kDa
Observed band size: 85 kDa
Additional bands at: 100 kDa, 45 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of ClC-4 in HUVEC cells, using ab75008 at a 1/500 dilution with (right image) or without (left image) the immunising peptide.
ab75008 has not yet been referenced specifically in any publications.