• Product name

    Anti-ClC-5 antibody [N237/24]
    See all ClC-5 primary antibodies
  • Description

    Mouse monoclonal [N237/24] to ClC-5
  • Host species

  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide aa 1-100 (N terminal). Cytoplasmic domain.

  • Positive control

    • IHC-P: FFPE human kidney tissue sections.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or  conjugation for your experiments, please contact orders@abcam.com.



Our Abpromise guarantee covers the use of ab174937 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function

    Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons. Important for normal acidification of the endosome lumen. May play an important role in renal tubular function.
  • Tissue specificity

    Kidney. Moderately expressed in aortic vascular smooth muscle and endothelial cells, and at a slightly higher level in the coronary vascular smooth muscle.
  • Involvement in disease

    Hypophosphatemic rickets, X-linked recessive
    Nephrolithiasis 2
    Nephrolithiasis 1
    Low molecular weight proteinuria with hypercalciuria and nephrocalcinosis
  • Sequence similarities

    Belongs to the chloride channel (TC 2.A.49) family. ClC-5/CLCN5 subfamily.
    Contains 2 CBS domains.
  • Post-translational

    Ubiquitinated by NEDD4L in the presence of albumin; which promotes endocytosis and proteasomal degradation.
  • Cellular localization

    Golgi apparatus membrane. Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride Channel 5 antibody
    • Chloride channel protein 5 antibody
    • Chloride channel voltage sensitive 5 antibody
    • Chloride transporter ClC-5 antibody
    • ClC-5 antibody
    • CLC5 antibody
    • CLCK2 antibody
    • CLCN5 antibody
    • CLCN5_HUMAN antibody
    • DENTS antibody
    • H(+)/Cl(-) exchange transporter 5 antibody
    • hCIC-K2 antibody
    • NPHL1 antibody
    • NPHL2 antibody
    • Voltage gated chloride ion channel CLCN5 antibody
    • XLRH antibody
    • XRN antibody
    see all


  • IHC image of ClC5 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab174937, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab174937 has not yet been referenced specifically in any publications.

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