• Product name
  • Description
    Rabbit polyclonal to CLC3
  • Host species
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Dog, Pig, Drosophila melanogaster, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to Human CLC3 (C terminal).


    (Peptide available as ab156309)

  • Positive control
    • Jurkat cell lysate, human brain tissue.
  • General notes

    Previously labelled as CLCN3. 



Our Abpromise guarantee covers the use of ab28736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1.5625e+006.
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 85 kDa.Can be blocked with CLC3 peptide (ab156309). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory.
  • Tissue specificity
    Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level).
  • Sequence similarities
    Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily.
    Contains 2 CBS domains.
  • Domain
    Isoform 2 contains a C-terminal PDZ-binding motif mediating the interaction with GOPC.
  • Post-translational
  • Cellular localization
    Membrane. Early endosome membrane. Late endosome membrane. Cytoplasmic vesicle > secretory vesicle membrane. Isoform 1 is localized mainly in early and late endosomes and Membrane. Early endosome membrane. Late endosome membrane. Golgi apparatus membrane. Isoform 2 partially colocalized with isoform 1 but is mainly enriched in the Golgi.
  • Information by UniProt
  • Database links
  • Alternative names
    • Chloride channel 3 antibody
    • Chloride channel protein 3 antibody
    • Chloride channel, voltage-sensitive 3 antibody
    • Chloride transporter ClC 3 antibody
    • Chloride transporter ClC-3 antibody
    • Chloride transporter ClC3 antibody
    • chloride voltage-gated channel 3 antibody
    • ClC 3 antibody
    • ClC-3 antibody
    • ClC3 antibody
    • CLCN 3 antibody
    • CLCN3 antibody
    • CLCN3_HUMAN antibody
    • DKFZp564I0463 antibody
    • H(+)/Cl(-) exchange transporter 3 antibody
    see all


  • Anti-CLC3 antibody (ab28736) at 1.25 µg/ml + HepG2 cell lysate

    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size: 85 kDa
    Observed band size: 93 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemisty analysis of human brain tissue labelling CLC3 with ab28736.

  • ICC/IF image of ab28736 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28736, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-CLC3 antibody (ab28736) at 2.5 µg/ml

    Lane 1 : HepG2 cell lysate
    Lane 2 : HepG2 cell lysate with blocking peptide at 2 µg/ml

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 85 kDa


This product has been referenced in:
  • Wang L  et al. The apoptotic effect of Zoledronic acid on the nasopharyngeal carcinoma cells via ROS mediated chloride channel activation. Clin Exp Pharmacol Physiol 45:1019-1027 (2018). Read more (PubMed: 29884989) »
  • He Z  et al. 3D map of the human corneal endothelial cell. Sci Rep 6:29047 (2016). Read more (PubMed: 27381832) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A


We have added the blocking peptide for ab28736 to our catalog:
CLCN3 peptide (https://www.abcam.com/clcn3-peptide-ab156309.html)

Read More


Thank you for contacting us.

We currently have three anti-CLCN3 products on our catalog. Unfortunately these are not phospho specific antibody and will recognize CLCN3 regardless of phosphorylation state. I can suggest two courses of action for detection of phosphorylated CLCN3. The first is to have a custom antibody created to specifically detected CLCN3 phosphorylated at S109. Our Epitomics group does provide custom rabbit monoclonal antibody services. Rabbits have a unique immune system which allows them to develop antibodies against “small epitopes” present in small molecules, lipids and polymers or subtle changes in epitopes such as post-translational modifications or single amino acid substitution. Rabbit monoclonal offer superior affinity as well. More information about our custom antibodies services may be found at: http://www.epitomics.com/services/ or by emailing service@epitomics.com.

You may also choose to use a non phospho specific antibody such as https://www.abcam.com/CIDE-C-antibody-ab77115.html, https://www.abcam.com/CIDE-C-antibody-ab16760.html or https://www.abcam.com/CIDE-C-antibody-ab102016.html in a WB system which incorporates a phos-tag acrylamide. These reagents specifically bind phosphate groups and can be incorporated into SDS-PAGE gels. These bind to phosphorylated proteins and hamper their progress through the gel. The more a protein is phosphorylated, the slower it will migrate and should resolve different phospho-species as individual bands. While this is an interesting system, we have not yet tested the phos-tag system and cannot guarantee its effectiveness.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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