Overview

  • Product name

  • Description

    Rabbit polyclonal to CLC7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N terminal region of Human CLCN7.

  • Positive control

    • CLO205 cell lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab136016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 89 kDa.

Target

  • Function

    Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the lysosome lumen.
  • Tissue specificity

    Brain, testis, muscle and kidney.
  • Involvement in disease

    Defects in CLCN7 are the cause of osteopetrosis autosomal recessive type 4 (OPTB4) [MIM:611490]; also known as infantile malignant osteopetrosis type 2. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood.
    Defects in CLCN7 are the cause of osteopetrosis autosomal dominant type 2 (OPTA2) [MIM:166600]; also known as autosomal dominant Albers-Schonberg disease or marble disease autosomal dominant. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTA2 is the most common form of osteopetrosis, occurring in adolescence or adulthood. It is characterized by sclerosis, predominantly involving the spine, the pelvis and the skull base.
  • Sequence similarities

    Belongs to the chloride channel (TC 2.A.49) family. ClC-7/CLCN7 subfamily.
    Contains 2 CBS domains.
  • Cellular localization

    Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chloride channel protein 7 antibody
    • CLC 7 antibody
    • ClC-7 antibody
    • ClC7 antibody
    • CLCN7 antibody
    • CLCN7_HUMAN antibody
    • FLJ26686 antibody
    • FLJ39644 antibody
    • FLJ46423 antibody
    • H(+)/Cl(-) exchange transporter 7 antibody
    • OPTA2 antibody
    • OPTB4 antibody
    see all

Images

  • Anti-CLC7 antibody (ab136016) at 1/500 dilution + COLO205 cell lysate at 30 µg

    Predicted band size: 89 kDa

References

ab136016 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Spleen and Liver)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
5 µg
Specification
Spleen and Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C

Dr. Helen Goodluck

Verified customer

Submitted Jan 23 2019

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