Recombinant
RabMAb

Anti-CLCA1 antibody [EPR12254-88] (ab180851)

Overview

  • Product name
    Anti-CLCA1 antibody [EPR12254-88]
    See all CLCA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12254-88] to CLCA1
  • Host species
    Rabbit
  • Specificity
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
    Database link: A8K7I4

  • Positive control
    • Human colon cancer and human small intestine lysates; human colon tissue; A431 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
IHC-P 1/4000.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

For unpurified use at 1/100 - 1/250.

WB 1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Function
    May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
  • Tissue specificity
    Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
  • Sequence similarities
    Belongs to the CLCR family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Glycosylated.
    The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
  • Cellular localization
    Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
  • Information by UniProt
  • Database links
  • Alternative names
    • CaCC antibody
    • CaCC-1 antibody
    • CACC1 antibody
    • Calcium dependent chloride channel 1 antibody
    • Calcium-activated chloride channel family member 1 antibody
    • Calcium-activated chloride channel protein 1 antibody
    • Calcium-activated chloride channel regulator 1 antibody
    • Chloride channel calcium activated family member 1 antibody
    • Chloride channel regulator 1 antibody
    • CLCA family member 1 chloride channel regulator antibody
    • CLCA1 antibody
    • CLCA1_HUMAN antibody
    • CLCRG1 antibody
    • GOB5 antibody
    • hCaCC-1 antibody
    • hCLCA1 antibody
    see all

Images

  • Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human small intestine tissue sections labeling CLCA1 with Purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/10000 dilution (purified) + Human small intestine lysates at 15 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 100 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/50000 dilution (purified)

    Lane 1 : Rat small intestine lysates
    Lane 2 : Mouse small intestine lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 100 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.

  • All lanes : Anti-CLCA1 antibody [EPR12254-88] (ab180851) at 1/1000 dilution (unpurified)

    Lane 1 : Human colon cancer lysates
    Lane 2 : Human small intestine lysates

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 100 kDa

References

This product has been referenced in:
  • Meldrum K  et al. Cerium dioxide nanoparticles exacerbate house dust mite induced type II airway inflammation. Part Fibre Toxicol 15:24 (2018). Read more (PubMed: 29792201) »
  • Herring CA  et al. Unsupervised Trajectory Analysis of Single-Cell RNA-Seq and Imaging Data Reveals Alternative Tuft Cell Origins in the Gut. Cell Syst 6:37-51.e9 (2018). Read more (PubMed: 29153838) »

See all 6 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Intestine
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 20% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 09 2018

Application
Western blot
Sample
Human Tissue lysate - other (Colon, Small Intestine)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
20 µg
Specification
Colon, Small Intestine
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 16 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Colonoids)
Permeabilization
Yes - 0.3% triton x-100
Specification
Colonoids
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up