Recombinant
RabMAb

Recombinant Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free (ab197059)

Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254-88] - BSA and Azide free
    See all CLCA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12254-88] to CLCA1 - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
    Database link: A8K7I4

  • Positive control

    • IHC-P: Human small intestine tissue.
  • General notes

    Ab197059 is the carrier-free version of ab180851. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab197059 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 100 kDa.
IHC-P Use at an assay dependent concentration.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
  • Tissue specificity

    Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
  • Sequence similarities

    Belongs to the CLCR family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Glycosylated.
    The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
  • Cellular localization

    Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
  • Information by UniProt
  • Database links

  • Alternative names

    • CaCC antibody
    • CaCC-1 antibody
    • CACC1 antibody
    • Calcium dependent chloride channel 1 antibody
    • Calcium-activated chloride channel family member 1 antibody
    • Calcium-activated chloride channel protein 1 antibody
    • Calcium-activated chloride channel regulator 1 antibody
    • Chloride channel calcium activated family member 1 antibody
    • Chloride channel regulator 1 antibody
    • CLCA family member 1 chloride channel regulator antibody
    • CLCA1 antibody
    • CLCA1_HUMAN antibody
    • CLCRG1 antibody
    • GOB5 antibody
    • hCaCC-1 antibody
    • hCLCA1 antibody
    see all

Images

  • Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling CLCA1 with purified ab180851 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling CLCA1 with Purified ab180851 at 1:100 dilution. Cells were fixed in 100% Methanol. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunofluorescence analysis of A431 cells labeling CLCA1 using unpurified ab180851 at 1/50 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CLCA1 using unpurified ab180851 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180851).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue sections labeling CLCA1 with purified ab180851 at 1:4000 dilution (0.07 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab180851).

References

ab197059 has not yet been referenced specifically in any publications.

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