Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254-88] (HRP)
    See all CLCA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12254-88] to CLCA1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Human CLCA1. The exact sequence is proprietary.
    Database link: A8K7I4

  • Positive control

    • WB: Small Intestine (Human) Tissue Lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab203736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 100 kDa).

Target

  • Function

    May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
  • Tissue specificity

    Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
  • Sequence similarities

    Belongs to the CLCR family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Glycosylated.
    The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
  • Cellular localization

    Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
  • Information by UniProt
  • Database links

  • Alternative names

    • CaCC antibody
    • CaCC-1 antibody
    • CACC1 antibody
    • Calcium dependent chloride channel 1 antibody
    • Calcium-activated chloride channel family member 1 antibody
    • Calcium-activated chloride channel protein 1 antibody
    • Calcium-activated chloride channel regulator 1 antibody
    • Chloride channel calcium activated family member 1 antibody
    • Chloride channel regulator 1 antibody
    • CLCA family member 1 chloride channel regulator antibody
    • CLCA1 antibody
    • CLCA1_HUMAN antibody
    • CLCRG1 antibody
    • GOB5 antibody
    • hCaCC-1 antibody
    • hCLCA1 antibody
    see all

Images

  • Anti-CLCA1 antibody [EPR12254-88] (HRP) (ab203736) at 1/1000 dilution + Small Intestine (Human) Tissue Lysate at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 100 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?


    Exposure time: 16 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab203736 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab203736 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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