Recombinant Anti-CLCA1 antibody [EPR12254] (ab174319)
Overview
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Product name
Anti-CLCA1 antibody [EPR12254]
See all CLCA1 primary antibodies -
Description
Rabbit monoclonal [EPR12254] to CLCA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human CLCA1.
Database link: A8K7I4 -
Positive control
- Human colon cancer and Human small intestine lysates; Human colon tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12254 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab174319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 100 kDa). | |
| IP | 1/10 - 1/100. |
Target
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Function
May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC. -
Tissue specificity
Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in. -
Sequence similarities
Belongs to the CLCR family.
Contains 1 VWFA domain. -
Post-translational
modificationsGlycosylated.
The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins. -
Cellular localization
Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli. - Information by UniProt
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Database links
- Entrez Gene: 1179 Human
- Omim: 603906 Human
- SwissProt: A8K7I4 Human
- Unigene: 194659 Human
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Alternative names
- CaCC antibody
- CaCC-1 antibody
- CACC1 antibody
see all
Images
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![Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)](https://www.abcam.com/ps/products/174/ab174319/Images/ab174319-199815-anti-clca1-antibody-epr12254-western-blot.jpg)
Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)All lanes : Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 100 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)](https://www.abcam.com/ps/products/174/ab174319/Images/ab174319-199816-anti-clca1-antibody-epr12254-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)](https://www.abcam.com/ps/products/174/ab174319/Images/ab174319-199817-anti-clca1-antibody-epr12254-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.
Protocols
Datasheets and documents
References
ab174319 has not yet been referenced specifically in any publications.
