Overview

  • Product name
    Anti-CLCA1 antibody [EPR12254]
    See all CLCA1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12254] to CLCA1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human CLCA1.
    Database link: A8K7I4

  • Positive control
    • Human colon cancer and Human small intestine lysates; Human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 100 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
    • Tissue specificity
      Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
    • Sequence similarities
      Belongs to the CLCR family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications
      Glycosylated.
      The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
    • Cellular localization
      Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
    • Information by UniProt
    • Database links
    • Alternative names
      • CaCC antibody
      • CaCC-1 antibody
      • CACC1 antibody
      • Calcium dependent chloride channel 1 antibody
      • Calcium-activated chloride channel family member 1 antibody
      • Calcium-activated chloride channel protein 1 antibody
      • Calcium-activated chloride channel regulator 1 antibody
      • Chloride channel calcium activated family member 1 antibody
      • Chloride channel regulator 1 antibody
      • CLCA family member 1 chloride channel regulator antibody
      • CLCA1 antibody
      • CLCA1_HUMAN antibody
      • CLCRG1 antibody
      • GOB5 antibody
      • hCaCC-1 antibody
      • hCLCA1 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution.

    • All lanes : Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution

      Lane 1 : Human colon cancer lysate
      Lane 2 : Human small intestine lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 100 kDa

    • Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.

    References

    ab174319 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174319.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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