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Signal Transduction Signaling Pathway Calcium Signaling Other
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RecombinantRabMAb

Recombinant Anti-CLCA1 antibody [EPR12254] (ab174319)

  • Datasheet
  • SDS
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Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)
  • Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)
  • Anti-CLCA1 antibody [EPR12254] (ab174319)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12254] to CLCA1
  • Suitable for: IHC-P, WB, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-CLCA1 antibody [EPR12254]
    See all CLCA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR12254] to CLCA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Recombinant fragment corresponding to Human CLCA1.
    Database link: A8K7I4

  • Positive control

    • Human colon cancer and Human small intestine lysates; Human colon tissue.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR12254
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Other
    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • Channels

Associated products

  • Alternative Versions

    • Anti-CLCA1 antibody [EPR12254] - BSA and Azide free (ab249816)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CLCA1 protein (ab114722)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174319 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 100 kDa).
IP
1/10 - 1/100.
Notes
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 100 kDa).
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      May be involved in mediating calcium-activated chloride conductance. May play critical roles in goblet cell metaplasia, mucus hypersecretion, cystic fibrosis and AHR. May be involved in the regulation of mucus production and/or secretion by goblet cells. Involved in the regulation of tissue inflammation in the innate immune response. May play a role as a tumor suppressor. Induces MUC5AC.
    • Tissue specificity

      Highly expressed in small intestine and colon namely in intestinal basal crypt epithelia and goblet cells, and appendix. Weakly expressed in uterus, testis and kidney. Expressed in the airways epithelium of both asthmatic andhealthy patients. Expressed in the bronchial epithelium, especially in mucus-producing goblet cells. Expressed in normal turbinate mucosa and nasal polyp. Expressed in.
    • Sequence similarities

      Belongs to the CLCR family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications

      Glycosylated.
      The 125-kDa product is processed and yields to two cell-surface-associated subunits, a 90-kDa protein and a group of 37-to 41-kDa proteins.
    • Cellular localization

      Secreted > extracellular space. Cell membrane. Protein that remains attached to the plasma membrane appeared to be predominantly localized to microvilli.
    • Target information above from: UniProt accession A8K7I4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1179 Human
      • Omim: 603906 Human
      • SwissProt: A8K7I4 Human
      • Unigene: 194659 Human
      • Alternative names

        • CaCC antibody
        • CaCC-1 antibody
        • CACC1 antibody
        • Calcium dependent chloride channel 1 antibody
        • Calcium-activated chloride channel family member 1 antibody
        • Calcium-activated chloride channel protein 1 antibody
        • Calcium-activated chloride channel regulator 1 antibody
        • Chloride channel calcium activated family member 1 antibody
        • Chloride channel regulator 1 antibody
        • CLCA family member 1 chloride channel regulator antibody
        • CLCA1 antibody
        • CLCA1_HUMAN antibody
        • CLCRG1 antibody
        • GOB5 antibody
        • hCaCC-1 antibody
        • hCLCA1 antibody
        see all

      Images

      • Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)
        Western blot - Anti-CLCA1 antibody [EPR12254] (ab174319)
        All lanes : Anti-CLCA1 antibody [EPR12254] (ab174319) at 1/10000 dilution

        Lane 1 : Human colon cancer lysate
        Lane 2 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 100 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CLCA1 antibody [EPR12254] (ab174319)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling CLCA1 with ab174319 at 1/100 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      • Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)
        Immunoprecipitation - Anti-CLCA1 antibody [EPR12254] (ab174319)

        Western blot analysis on immunoprecipitation pellet from Human colon cancer lysate using ab174319 at 1/10 dilution.

      • Anti-CLCA1 antibody [EPR12254] (ab174319)
        Anti-CLCA1 antibody [EPR12254] (ab174319)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab174319? Please let us know so that we can cite the reference in this datasheet.

      ab174319 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CLCA1 antibody [EPR12254]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Rhesus monkey Tissue sections (stomach)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate pH6.0
      Permeabilization
      No
      Specification
      stomach
      Blocking step
      Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 12 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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