Overview

  • Product name
  • Description
    Rabbit polyclonal to CLCA4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: WVGMVHFDST ATIVNKLIQI KSSDERNTLM AGLPTYPLGG TSICSGIKYA FQVIGELHSQ LDGSEVLLLT DGEDNTASSC IDEV, corresponding to amino acids 56-139 of Human CLCA4.

  • Positive control
    • Human duodenum.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.02% Sodium azide
    Constituents: 60% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    May be involved in mediating calcium-activated chloride conductance.
  • Tissue specificity
    Primarily expressed in the digestive tract, mainly in colon. Detected in smaller amounts in brain, urogenital organs, testis, and salivary and mammary glands. Highly expressed in the epithelial layer and submucosal gland of the inferior turbinate mucosa. Lower levels in the epithelial layer of nasal polyp.
  • Sequence similarities
    Belongs to the CLCR family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Processed at a monobasic residue to yield a larger N-terminal and a smaller C-terminal cleavage products of 90 to 110 and 30 to 35 kDa, respectively.
  • Cellular localization
    Cell membrane. Apical cell membrane. Secreted. The C-terminus 30 kDa form is anchored to the membrane (By similarity). The N-terminus 110 kDa form is released from the membrane triggered by an unknown stimulus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 30 kDa form antibody
    • CaCC-2 antibody
    • Calcium-activated chloride channel family member 4 antibody
    • Calcium-activated chloride channel protein 2 antibody
    • Calcium-activated chloride channel regulator 4 antibody
    • Clca4 antibody
    • CLCA4_HUMAN antibody
    • hCaCC-2 antibody
    • hCLCA4 antibody
    see all

Images

  • ab121524, at 1/10 dilution, staining human duodenum by immunohistochemistry on paraffin embebbed tissues (IHC-P).

References

ab121524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

Thank you very much for your interest in ab121524.
To our knowledge, ab121524 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab121524 now, test it in WB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody off your next Abcam order.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab121524 in WB. I will then send a discount code. This code must be issued before purchasing ab121524 so please wait for my reply before ordering.
2. Purchase ab121524 either by phone, fax, or online (www.abcam.com).
3. Test it in WB.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab121524 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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