Overview

  • Product name

  • Description

    Rabbit polyclonal to CLCA4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CLCA4 (internal sequence).
    Database link: Q14CN2

  • Positive control

    • 293T cell lysate. Human liver cancer tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab197347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/100 - 1/500. Predicted molecular weight: 101 kDa.

Target

  • Function

    May be involved in mediating calcium-activated chloride conductance.
  • Tissue specificity

    Primarily expressed in the digestive tract, mainly in colon. Detected in smaller amounts in brain, urogenital organs, testis, and salivary and mammary glands. Highly expressed in the epithelial layer and submucosal gland of the inferior turbinate mucosa. Lower levels in the epithelial layer of nasal polyp.
  • Sequence similarities

    Belongs to the CLCR family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications

    Processed at a monobasic residue to yield a larger N-terminal and a smaller C-terminal cleavage products of 90 to 110 and 30 to 35 kDa, respectively.
  • Cellular localization

    Cell membrane. Apical cell membrane. Secreted. The C-terminus 30 kDa form is anchored to the membrane (By similarity). The N-terminus 110 kDa form is released from the membrane triggered by an unknown stimulus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 30 kDa form antibody
    • CaCC-2 antibody
    • Calcium-activated chloride channel family member 4 antibody
    • Calcium-activated chloride channel protein 2 antibody
    • Calcium-activated chloride channel regulator 4 antibody
    • Clca4 antibody
    • CLCA4_HUMAN antibody
    • hCaCC-2 antibody
    • hCLCA4 antibody
    see all

Images

  • Anti-CLCA4 antibody (ab197347) at 1/200 dilution + 293T cell lysate at 40 µg

    Secondary
    Anti-rabbit IgG at 1/8000 dilution

    Predicted band size: 101 kDa


    Exposure time: 30 seconds


    Gel: 8%SDS-PAGE.

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue sections labeling CLCA4 with ab197347 at 1/10 dilution.

References

This product has been referenced in:

  • Chen H  et al. Calcium-Activated Chloride Channel A4 (CLCA4) Plays Inhibitory Roles in Invasion and Migration Through Suppressing Epithelial-Mesenchymal Transition via PI3K/AKT Signaling in Colorectal Cancer. Med Sci Monit 25:4176-4185 (2019). Read more (PubMed: 31164625) »
  • Liu Z  et al. CLCA4 inhibits cell proliferation and invasion of hepatocellular carcinoma by suppressing epithelial-mesenchymal transition via PI3K/AKT signaling. Aging (Albany NY) 10:2570-2584 (2018). Read more (PubMed: 30312171) »
See all 3 Publications for this product

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