Anti-CLCNKB antibody (ab236733)
Key features and details
- Rabbit polyclonal to CLCNKB
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CLCNKB antibody
See all CLCNKB primary antibodies -
Description
Rabbit polyclonal to CLCNKB -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human CLCNKB aa 500 to the C-terminus.
Database link: P51801 -
Positive control
- WB: HeLa whole cell lysate; Rat kidney tissue lysate. IHC-P: Human breast cancer tissue. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500.
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WB |
1/500 - 1/5000. Predicted molecular weight: 75 kDa.
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ICC/IF |
1/50 - 1/200.
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Notes |
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IHC-P
1/200 - 1/500. |
WB
1/500 - 1/5000. Predicted molecular weight: 75 kDa. |
ICC/IF
1/50 - 1/200. |
Target
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Function
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms. -
Tissue specificity
Expressed predominantly in the kidney. -
Involvement in disease
Defects in CLCNKB are the cause of Bartter syndrome type 3 (BS3) [MIM:607364]; also known as classic Bartter syndrome. It is an autosomal recessive form of often severe intravascular volume depletion due to renal salt-wasting associated with low blood pressure, hypokalemic alkalosis, hypercalciuria, and normal serum magnesium levels.
Defects in CLCNKB are a cause of Bartter syndrome type 4B (BS4B) [MIM:613090]. A digenic, recessive disorder characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. Bartter syndrome type 4B is associated with sensorineural deafness. -
Sequence similarities
Belongs to the chloride channel (TC 2.A.49) family. CLCNKB subfamily.
Contains 2 CBS domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1188 Human
- Entrez Gene: 79430 Rat
- Omim: 602023 Human
- SwissProt: P51801 Human
- SwissProt: P51802 Rat
- Unigene: 352243 Human
- Unigene: 10339 Rat
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Alternative names
- Bartter syndrome type 3 antibody
- Chloride channel Kb antibody
- Chloride channel kidney B antibody
see all
Images
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Paraffin-embedded human breast cancer tissue stained for CLCNKB using ab236733 at 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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All lanes : Anti-CLCNKB antibody (ab236733) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Rat kidney tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 75 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells labeling CLCNKB (Green) using ab236733 at 1/100 dilution in ICC/IF, followed by Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L). Counterstained with DAPI.
The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236733 has not yet been referenced specifically in any publications.