Overview

  • Product name

    Anti-CLDN16/Claudin-16 antibody
    See all CLDN16/Claudin-16 primary antibodies
  • Description

    Rabbit polyclonal to CLDN16/Claudin-16
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CLDN16/Claudin-16 aa 6-33 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9Y5I7

  • Positive control

    • WB: MDA-MB-435 cell lysate. IHC-P: Human kidney and testis tissues. Flow Cyt: MDA-MB-435 cells.
  • General notes

     This product was previously labelled as CLDN16

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab230440 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors.
  • Tissue specificity

    Kidney-specific, including the thick ascending limb of Henle (TAL).
  • Involvement in disease

    Defects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3) [MIM:248250]; also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia.
  • Sequence similarities

    Belongs to the claudin family.
  • Cellular localization

    Cell junction > tight junction. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Claudin 16 antibody
    • Claudin-16 antibody
    • CLD16_HUMAN antibody
    • CLDN 16 antibody
    • Cldn16 antibody
    • Paracellin 1 antibody
    • Paracellin-1 antibody
    • PCLN-1 antibody
    • PCLN1 antibody
    see all

Images

  • Anti-CLDN16/Claudin-16 antibody (ab230440) at 1/1000 dilution + MDS-MB-435 (human ductal carcinoma cell line) cell lysate at 35 µg

    Predicted band size: 34 kDa

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for CLDN16/Claudin-1 using ab230440 at 1/100 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for CLDN16/Claudin-1 using ab230440 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of MDA-MB-435 (human ductal carcinoma cell line) cells labeling CLDN16/Claudin-1 with ab230440 at 1/10 dilution (green) compared with a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used.

References

ab230440 has not yet been referenced specifically in any publications.

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