Key features and details
- Rabbit polyclonal to CLDN16/Claudin-16
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CLDN16/Claudin-16 antibody
See all CLDN16/Claudin-16 primary antibodies
DescriptionRabbit polyclonal to CLDN16/Claudin-16
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human CLDN16/Claudin-16 aa 6-33 (N terminal) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9Y5I7
- WB: MDA-MB-435 cell lysate. IHC-P: Human kidney and testis tissues. Flow Cyt: MDA-MB-435 cells.
This product was previously labelled as CLDN16
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230440 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 34 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.|
FunctionPlays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors.
Tissue specificityKidney-specific, including the thick ascending limb of Henle (TAL).
Involvement in diseaseDefects in CLDN16 are the cause of hypomagnesemia type 3 (HOMG3) [MIM:248250]; also known as familial hypomagnesemia with hypercalciuria and nephrocalcinosis (FHHNC). HOMG3 is a progressive renal disease characterized by primary renal magnesium wasting with hypomagnesemia, hypercalciuria and nephrocalcinosis. Recurrent urinary tract infections and kidney stones are often observed. In spite of hypercalciuria, patients do not show hypocalcemia.
Sequence similaritiesBelongs to the claudin family.
Cellular localizationCell junction > tight junction. Cell membrane.
- Information by UniProt
- Claudin 16 antibody
- Claudin-16 antibody
- CLD16_HUMAN antibody
Anti-CLDN16/Claudin-16 antibody (ab230440) at 1/1000 dilution + MDS-MB-435 (human ductal carcinoma cell line) cell lysate at 35 µg
Predicted band size: 34 kDa
Formalin-fixed, paraffin-embedded human kidney tissue stained for CLDN16/Claudin-1 using ab230440 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human testis tissue stained for CLDN16/Claudin-1 using ab230440 at 1/100 dilution in immunohistochemical analysis.
Flow cytometric analysis of MDA-MB-435 (human ductal carcinoma cell line) cells labeling CLDN16/Claudin-1 with ab230440 at 1/10 dilution (green) compared with a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230440 has not yet been referenced specifically in any publications.