Recombinant
RabMAb

Recombinant Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821)

Overview

  • Product name

    Anti-cleaved C-terminal GSDMD antibody [EPR20885-203]
    See all cleaved C-terminal GSDMD primary antibodies
  • Description

    Rabbit monoclonal [EPR20885-203] to cleaved C-terminal GSDMD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human cleaved C-terminal GSDMD aa 250-350. The exact sequence is proprietary.
    Database link: P57764

  • Positive control

    • WB: SiHa (treated with 5 µg LPS/10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR20885-203
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 53 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Gasdermin-D, N-terminal: Promotes pyroptosis in response to microbial infection and danger signals. Produced by the cleavage of gasdermin-D by inflammatory caspases CASP1 or CASP4 in response to canonical, as well as non-canonical (such as cytosolic LPS) inflammasome activators (PubMed:26375003, PubMed:26375259, PubMed:27418190). After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, including monophosphorylated phosphatidylinositols, such as phosphatidylinositol 4-phosphate, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate, as well as phosphatidylinositol (3,4,5)-bisphosphate, and more weakly to phosphatidic acid and phosphatidylserine (PubMed:27281216). Homooligomerizes within the membrane and forms pores of 10 - 15 nanometers (nm) of inner diameter, possibly allowing the release of mature IL1B and triggering pyroptosis (PubMed:27418190, PubMed:27281216). Exhibits bactericidal activity. Gasdermin-D, N-terminal released from pyroptotic cells into the extracellular milieu rapidly binds to and kills both Gram-negative and Gram-positive bacteria, without harming neighboring mammalian cells, as it does not disrupt the plasma membrane from the outside due to lipid-binding specificity (PubMed:27281216). Under cell culture conditions, also active against intracellular bacteria, such as Listeria monocytogenes (By similarity). Strongly binds to bacterial and mitochondrial lipids, including cardiolipin. Does not bind to unphosphorylated phosphatidylinositol, phosphatidylethanolamine nor phosphatidylcholine (PubMed:27281216).
    • Tissue specificity

      Expressed in the suprabasal cells of esophagus, as well as in the isthmus/neck, pit, and gland of the stomach, suggesting preferential expression in differentiating cells.
    • Sequence similarities

      Belongs to the gasdermin family.
    • Domain

      Intramolecular interactions between N- and C-terminal domains may be important for autoinhibition in the absence of cleavage by inflammatory caspases CASP1 or CASP4. The intrinsic pyroptosis-inducing activity is carried by gasdermin-D, N-terminal, that is released upon cleavage by inflammatory caspases.
    • Post-translational
      modifications

      Cleavage at Asp-275 by CASP1 (mature and uncleaved precursor forms) or CASP4 relieves autoinhibition and is sufficient to initiate pyroptosis.
    • Cellular localization

      Cell membrane. Secreted. Released in the extracellular milieu following pyroptosis and Cytoplasm, cytosol. Inflammasome. In response to a canonical inflammasome stimulus, such as nigericin, recruited to NLRP3 inflammasone with similar kinetics to that of uncleaved CASP1 precursor.
    • Information by UniProt
    • Database links

    • Alternative names

      • C-terminal antibody
      • Gasdermin domain-containing protein 1 antibody
      • Gasdermin-D antibody
      • GSDMD antibody
      • GSDMD-CT antibody
      • GSDMD-NT antibody
      • GSDMD_HUMAN antibody
      see all

    Images

    • All lanes : Anti-cleaved C-terminal GSDMD antibody [EPR20885-203] (ab227821) at 1/1000 dilution

      Lane 1 : SiHa (human cervical squamous cell carcinoma), cell lysate plus concentrated cell supernatant
      Lane 2 : SiHa (treated with 5 µg LPS/ 10E6 cells by electroporation for 2h.), cell lysate plus concentrated supernatant.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/5000 dilution

      Predicted band size: 53 kDa
      Observed band size: 22 kDa
      why is the actual band size different from the predicted?


      Exposure time: 5 minutes


      The image is kindly provided by Dr Feng Shao.

      The molecular weight observed is consistent with literature (PMID: 26375003).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab227821 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab227821.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up