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  1. Link

    cleaved-caspase-3-antibody-ab2302.pdf

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Anti-Cleaved Caspase-3 antibody (ab2302)

  • Datasheet
  • SDS
Reviews (58)Q&A (37)References (986)

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Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
  • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
  • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)

Key features and details

  • Rabbit polyclonal to Cleaved Caspase-3
  • Suitable for: WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Cleaved Caspase-3 antibody [E83-77] (ab32042)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Cleaved Caspase-3 antibody
    See all Cleaved Caspase-3 primary antibodies
  • Description

    Rabbit polyclonal to Cleaved Caspase-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Synthetic peptide corresponding to Human Cleaved Caspase-3. Synthetic peptide mapping to the N-terminus adjacent to the cleavage site of human caspase-3
    Database link: P42574
    (Peptide available as ab38283)

  • Positive control

    • WB: HeLa (treated with staurosporine) whole cell lysate. Camptothecin treated Jurkat whole cell lysate. Cleaved Caspase 3 recombinant protein.
  • General notes

    This product is manufactured by BioVision, an Abcam company and was previously called 3015 Anti-Caspase-3 (Active) Polyclonal Antibody.

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.03% Proclin 300
    Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.146% EDTA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Caspases are synthesized as inactive pro-enzymes that are processed to active form in cells undergoing apoptosis. Caspase 3 has been extensively studied and implicated to play an important role in apoptosis. Active caspase 3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP). This affinity purified antibody recognizing the active forms of caspase-3 provides a new tool for identifying apoptotic cell populations in both tissue sections and cultured cells.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Other
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases

Associated products

  • Assay kits

    • Caspase-3 Assay Kit (Colorimetric) (ab39401)
    • Cleaved Caspase-3 Staining Kit (FITC) (ab65613)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Cleaved Caspase-3 protein (Active) (ab52101)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
    • Anti-Cytochrome C antibody [7H8.2C12] (ab13575)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (16)
1/50 - 1/500. Detects a band of approximately 17 kDa.
Notes
WB
1/50 - 1/500. Detects a band of approximately 17 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • Tissue specificity

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession P42574 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 836 Human
    • Omim: 600636 Human
    • SwissProt: P42574 Human
    • Unigene: 141125 Human
    • Alternative names

      • active caspase 3 antibody
      • Apopain antibody
      • CASP 3 antibody
      • CASP-3 antibody
      • CASP3 antibody
      • CASP3_HUMAN antibody
      • Caspase 3 antibody
      • Caspase-3 subunit p12 antibody
      • CPP 32 antibody
      • CPP-32 antibody
      • CPP32B antibody
      • Cysteine protease CPP32 antibody
      • PARP cleavage protease antibody
      • Protein Yama antibody
      • SCA-1 antibody
      • SCA1 antibody
      • SREBP cleavage activity 1 antibody
      • Yama antibody
      see all

    Images

    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      All lanes : Anti-Cleaved Caspase-3 antibody (ab2302)

      Lane 1 : Untreated Jurkat cell line (human T cell leukemia cell line from peripheral blood)
      Lane 2 : Camptothecin treated Jurkat cell line (human T cell leukemia cell line from peripheral blood)

      Lysates/proteins at 60 µg per lane.
    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      All lanes : Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (2 uM Staurosporine, 4Hr) at 20 µg
      Lane 2 : HeLa whole cell lysate (untreated) at 20 µg
      Lane 3 : Cleaved Caspase 3 (recombinant protein) at 0.1 µg

      Secondary
      All lanes : 800CW Goat Anti-Rabbit IgG at 1/10000 dilution

      Performed under reducing conditions.

      Additional bands at: 17 kDa (possible mature (processed) protein), 24 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

    • Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Western blot - Anti-Cleaved Caspase-3 antibody (ab2302)
      Anti-Cleaved Caspase-3 antibody (ab2302) at 1 µg/ml + Camptothecin
      (2 µM) treated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Protocols

    • Recommended protocol for WB with ab2302

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (986)

    Publishing research using ab2302? Please let us know so that we can cite the reference in this datasheet.

    ab2302 has been referenced in 986 publications.

    • Liu F  et al. LncRNA LIFR-AS1 overexpression suppressed the progression of serous ovarian carcinoma. J Clin Lab Anal N/A:e25470 (2022). WB ; Human . PubMed: 35778954
    • Wang J  et al. MiR-122 Participates in Oxidative Stress and Apoptosis in STZ-Induced Pancreatic ß Cells by Regulating PI3K/AKT Signaling Pathway. Int J Endocrinol 2021:5525112 (2021). PubMed: 34054947
    • Shui S  et al. Non-enzymatic lipid peroxidation initiated by photodynamic therapy drives a distinct ferroptosis-like cell death pathway. Redox Biol 45:102056 (2021). PubMed: 34229160
    • Wu Q  et al. Histone deacetylase 1 facilitates aerobic glycolysis and growth of endometrial cancer. Oncol Lett 22:721 (2021). PubMed: 34429761
    • Zhou XH  et al. Osthole induces apoptosis of the HT-29 cells via endoplasmic reticulum stress and autophagy. Oncol Lett 22:726 (2021). PubMed: 34429766
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    11-20 of 95 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cleaved Caspase-3 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (uterus)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: high Target retrival solution
    Permeabilization
    No
    Specification
    uterus
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    FRAU. Ines Kleiber-Schaaf

    Verified customer

    Submitted May 29 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-active Caspase-3 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (pancreas)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    Yes - TBS, 0.4% TX100
    Specification
    pancreas
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 17 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (cartilage/bone)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 100ug/ml proteinase K
    Permeabilization
    No
    Specification
    cartilage/bone
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 17 2019

    Western blot abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (neuroblastoma)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    40 µg
    Specification
    neuroblastoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 21 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Fibroblast from human tumor tissue)
    Permeabilization
    Yes - 0.1%Triton X100 in PBS
    Specification
    Fibroblast from human tumor tissue
    Blocking step
    DakoCytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Hongwei Shao

    Verified customer

    Submitted Dec 17 2018

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-active Caspase-3 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (mouse melanoma cells injected into mouse brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton-X
    Specification
    mouse melanoma cells injected into mouse brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Yona Goldshmit

    Verified customer

    Submitted Dec 12 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-active Caspase-3 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neurons)
    Permeabilization
    Yes - triton X 100 1%
    Specification
    neurons
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 11 2018

    Immunohistochemistry free floating abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 31 2018

    Immunohistochemistry free floating abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 31 2018

    IHC - Wholemount abreview for Anti-active Caspase-3 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Rat Tissue (embryonic lung explant)
    Specification
    embryonic lung explant
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 30 2018

    11-20 of 95 Abreviews or Q&A

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