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Wants to know what the specificity reference is and what it means.
Asked on Jun 29 2012
Thank you for contacting us yesterday and your interest in our products.
I have had a look at the reference we discussed yesterday, mentioned in the specificity section of the anti-active Caspase 3 antibody (ab2302):
Bhoopathi P et al. Blockade of tumor growth due to matrix metalloproteinase-9 inhibition is mediated by sequential activation of 1-integrin, ERK, and NF-kappaB. J Biol Chem 283:1545-52 (2008).PubMed: 17991734
This reference is specifically mentioned as it has used the antibody and detected both the pro and active forms of Caspase 3. This is in contrast to the conditions which we have used in our Western blotting experiments and several other references where only the p17 active Caspase 3 fragment was detected.
The mention of this reference is simply to illustrate that under certain experimental conditions the pro form may be detected, but we have found the antibody to preferentially recognise the pro-form.
I hope this information has been of help. If you have any further questions, please do not hesitate to contact us again.
Answered on Jun 29 2012