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We have been testing an antibody anti-active Caspase 3 that was purchased from Abcam (ab2302). When we tested the positive control that you suggest (Camptothecin 2 µM treated Jurkat cells) we obtained a very weak signal using the antibody at the concentration 1:100. We already tried several protein concentrations as well as different denaturation temperatures and transfer times. The results were always as you can see in the picture or even worst. Hence, we would like to know if you have any suggestion.
Asked on Aug 27 2012
Thank you for contacting us.
I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am not aware of any problems with this antibody.
I would like however to investigate this in more details. Can you please send me the technical details of the protocol as well? Additionally, please send me the order number or the date on which the antibody has been ordered. This will allow me to identify the vial.
I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results.
If the productwould be damaged orfaulty,we would be happy to replace or refund the antibody.
I look forward to receiving your reply.
Answered on Aug 27 2012