Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (BRAIN)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
BRAIN
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
FORMALIN
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: BSA
Secondary antibody
Dilution
1/50
Name
Non-Abcam antibody was used: VECTASTAIN® Elite® ABC HRP Kit (Peroxidase, Univer
Additional data
Additional Notes
For IHC, I used (VECTASTAIN® Elite® ABC HRP Kit (Peroxidase, Universal) 1:50 normal horse serum and 1:50 biotinylated Universal Antibody 1:50, mixed well in 1X TBST
Abcam user community
Verified customer
Submitted Nov 01 2019