Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Dorsal root ganglia neurons)
Permeabilization
No
Specification
Dorsal root ganglia neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: AlexaFluor 350 Donkey anti-Rabbit IgG (H+L) Highly
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other
Additional data
Additional Notes
Mouse dorsal root ganglia neurons co-cultured with mouse bone marrow derived macrophages (cultured for 1 week prior) overnight. Cover slips were fixed in warm 2% PFA at 37°C for 30 minutes before being blocked in 3% BSA, 0.2% Triton in 1x PBS for 30 minutes at room temperature. Cover slips were stained with antibodies against Iba1 (red), Anti-ßIII Tubulin (green) and Caspase 3 (blue) for 1 hour, followed by secondary staining with AlexaFluor antibodies for 1 hour.
Abcam response
Abcam has not tested the antibody in Mouse samples nor ICC and thus can't say how it should have performed. The antibody Lot tested is approximately 12 years old.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Zoe Hore
Verified customer
Submitted Dec 10 2019