Immunocytochemistry abreview for Anti-Cleaved Caspase-3 antibody

Immunocytochemistry

Mouse Cultured Cells (Dorsal root ganglia neurons)

No

Dorsal root ganglia neurons

BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C

Paraformaldehyde

1 hour(s) and 0 minute(s) · Temperature: 23°C

1/200

1/1000

Non-Abcam antibody was used: AlexaFluor 350 Donkey anti-Rabbit IgG (H+L) Highly
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other

Mouse dorsal root ganglia neurons co-cultured with mouse bone marrow derived macrophages (cultured for 1 week prior) overnight. Cover slips were fixed in warm 2% PFA at 37°C for 30 minutes before being blocked in 3% BSA, 0.2% Triton in 1x PBS for 30 minutes at room temperature. Cover slips were stained with antibodies against Iba1 (red), Anti-ßIII Tubulin (green) and Caspase 3 (blue) for 1 hour, followed by secondary staining with AlexaFluor antibodies for 1 hour.

Abcam has not tested the antibody in Mouse samples nor ICC and thus can't say how it should have performed. The antibody Lot tested is approximately 12 years old.