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Immunocytochemistry abreview for Anti-Cleaved Caspase-3 antibody

Inconclusive
Abreviews
Abreviews
Go to datasheet (ab2302)
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Dorsal root ganglia neurons)
Permeabilization
No
Specification
Dorsal root ganglia neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C
Dilution
1/200

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: AlexaFluor 350 Donkey anti-Rabbit IgG (H+L) Highly
Host species: Donkey
Clonality: Polyclonal
Conjugation: Other

Additional data

Additional Notes
Mouse dorsal root ganglia neurons co-cultured with mouse bone marrow derived macrophages (cultured for 1 week prior) overnight. Cover slips were fixed in warm 2% PFA at 37°C for 30 minutes before being blocked in 3% BSA, 0.2% Triton in 1x PBS for 30 minutes at room temperature. Cover slips were stained with antibodies against Iba1 (red), Anti-ßIII Tubulin (green) and Caspase 3 (blue) for 1 hour, followed by secondary staining with AlexaFluor antibodies for 1 hour.
Abcam response
Abcam has not tested the antibody in Mouse samples nor ICC and thus can't say how it should have performed. The antibody Lot tested is approximately 12 years old.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MISS. Zoe Hore

Verified customer

Submitted Dec 10 2019

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