Overview

  • Product name

    Anti-Cleaved Caspase-3 antibody
    See all Cleaved Caspase-3 primary antibodies
  • Description

    Rabbit polyclonal to Cleaved Caspase-3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Cow, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Cleaved Caspase-3 aa 167-175 conjugated to keyhole limpet haemocyanin.
    Sequence:

    ELDCGIETD


    Database link: P42574

  • Positive control

    • Human epitheloid carcinoma HeLa cell line, treated with staurosporine and Human recombinant caspase-3, active.

Properties

Applications

Our Abpromise guarantee covers the use of ab49822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500.
ICC/IF 1/1000.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-
    -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
  • Tissue specificity

    Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
  • Sequence similarities

    Belongs to the peptidase C14A family.
  • Post-translational
    modifications

    Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
    S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • active caspase 3 antibody
    • Apopain antibody
    • CASP 3 antibody
    • CASP-3 antibody
    • CASP3 antibody
    • CASP3_HUMAN antibody
    • Caspase 3 antibody
    • Caspase-3 subunit p12 antibody
    • CPP 32 antibody
    • CPP-32 antibody
    • CPP32B antibody
    • Cysteine protease CPP32 antibody
    • PARP cleavage protease antibody
    • Protein Yama antibody
    • SCA-1 antibody
    • SCA1 antibody
    • SREBP cleavage activity 1 antibody
    • Yama antibody
    see all

Images

  • Anti-Cleaved Caspase-3 antibody (ab49822) at 1/1000 dilution
    Secondary
    Goat Anti-Rabbit IgG, Peroxidase conjugate

    Developed using the ECL technique.

    Observed band size: 17 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Zhong KL  et al. Isosteviol Sodium Protects Neural Cells Against Hypoxia-Induced Apoptosis Through Inhibiting MAPK and NF-?B Pathways. J Stroke Cerebrovasc Dis 28:175-184 (2019). Read more (PubMed: 30539754) »
  • Gong G  et al. Tanshinone IIA alleviates oxidative damage after spinal cord injury in vitro and in vivo through up-regulating miR-124. Life Sci 216:147-155 (2019). Read more (PubMed: 30468834) »
See all 65 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse RPE)
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris gel)
Loading amount
20 µg
Treatment
3-mo cigarette smoking
Specification
mouse RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 28 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat Cell Line)
Gel Running Conditions
Reduced Denaturing (4-12% bis-tris gel)
Loading amount
10 µg
Treatment
cytochrome c
Specification
Jurkat Cell Line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 28 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Feb 05 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Sheep Tissue sections (Thymus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization
No
Specification
Thymus
Blocking step
(agent) for 10 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 15 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Ferret Tissue sections (Small Intestines)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial Retrieval Solution
Permeabilization
No
Specification
Small Intestines
Fixative
Formaldehyde

Fabian Lean

Verified customer

Submitted Jul 19 2016

Question
Answer

This antibody is specific to the active form of caspase 3.

Read More

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